摘要
目的 探讨胶原酶诱导脑出血模型小鼠血肿周围组织ATP结合盒转运体A1（ATP binding cassette transporter A1,ABCA1）表达.方法 立体定向注射Ⅳ型胶原酶建立小鼠尾状壳核脑出血模型,在模型制作后24 h、48 h和72 h利用行为学评分评估神经功能缺损,尼氏染色检测血肿周围神经元形态,免疫组化染色及蛋白质印迹分析检测血肿周围ABCA1表达.结果 血肿周围神经元尼氏小体减少、萎缩、坏死,且随着时间推移而加重.免疫组化染色和蛋白质印迹分析均显示血肿周围组织ABCA1表达水平显著高于假手术组（P均〈0.05）,且表达水平随着时间的推移显著增高（P均〈0.05）.结论 ABCA1在脑出血后表达上调,提示其可能参与脑出血的病理学过程.
Objective To investigate the ATP binding cassette transporter A1 （ABCA1） expression in perihematomal tissue of mouse cerebral hemorrhage model induced by collagenase. Methods Stereotactic injection of type Ⅳ collagenase was used to induce a model of caudate putamen intracerebral hemorrhage in mice. The behavioral scores were use to assess neurological deficits at 24 h, 48 h and 72 h after model making. Neisserian staining was used to detect the morphology of neurons around hematomas.Immunohistochemical staining and Western blot analysis were used to detect the expression of ABCA1 around hematomas. Results Nissl bodies reduction, atrophy, necrosis of perihematomal neurons were observed and aggravated over time. Immunohistochemical staining and Western blot analysis showed that the expression level of ABCA1 in the perihematomal tissue was significantly higher than that in the sham operation group （all P 〈 0. 05）, and the expression level increased significantly with time （all P 〈 0. 05 ）.Conclusion ABCA1 was up-regulated after cerebral hemorrhage, suggesting that it might be involved in the pathological process of cerebral hemorrhage.
作者
唐向明
陈子旋
王英歌
陈宏梅
徐将
段作伟
王小洪
梁景岩
张新江
Tang Xiangming;Chen Zixuan;Wang Y＇mgge;Chen Hongmei;Xu Jiang;Duan Zuowei;Wang Xiaohong;Liang Jingyan;Zhang Xinjiang(Medical College of Yangzhou University,Yangzhou 225000,China;School of Nursing,Yangzhou University,Yangzhou 225000,China;Department of Neurology,the Affiliated Hospital of Yangzhou University,Yangzhou 225000,China)
出处
《国际脑血管病杂志》
2018年第9期689-695,共7页
International Journal of Cerebrovascular Diseases
基金
国家重点研发项目（2016YFE0126000）
国家自然科学基金（81570392）
江苏省卫计委项目（QNRC2016353）
江苏省高等学校大学生创新训练计划重点项目（201811117005Z）
关键词
脑出血
ATP结合盒转运体1
疾病模型
动物
小鼠
Cerebral Hemorrhage
ATP Binding Cassette Transporter 1
Disease Models
Animal
Mice
