摘要
目的:建立鼻窦炎颗粒中木兰脂素的鉴别与含量测定的方法。方法:采用TLC法对鼻窦炎颗粒中木兰脂素进行定性鉴别;HPLC法进行含量测定,色谱柱Symmetry C18(150 mm×6 mm,5μm),流动相:乙腈-水(45∶55),流速:1.0 mL/min,进样量:10μL,检测波长:203 nm。结果:TLC定性鉴别:阴性对照无干扰,斑点清晰;HPLC法含量测定:木兰脂素在1～50μg/mL范围内与峰面积呈现良好线性关系(R2=0.9996),平均回收率为99.50%。结论:TLC、HPLC方法简单、准确,灵敏度高,重复性好,可用于鼻窦炎中木兰脂素的鉴别和含量测定。
Objective： To set up the methods about identify and determination the content of Magnolin in Bidouyan granules. Methods： By TLC, Magnolin in Bidouyan granules; Symmetry C18（5 μm, 6 mm×150 mm）was the chromatographic column. The mobile phase was acetonitrile-water（45∶55）. The flow rate was 1.0 mL/min, and the detection wavelength was 203 nm. The injection volume was 10 μL. Results： In the qualitative identification,the clear spots showed good resolution, and TLC spots specific without interference from negative control. Within the range from 1 to 50 μg/mL, Magnolin presented a good linear relationship with the peak areas（R2=0.9996）.The average recovery was 99.50%. Conclusion： The methods of TLC and HPLC are simple and accurate with good specificity and reproducibility. They can be used for the identify and determination the content of Magnolin in Bidouyan granules.
作者
田蜜
陈芳
贺建昌
TIAN Mi;CHEN Fang;HE Jian-chang(Kumning General Hospital of Chengdu Military Command,Kunming Yunnan 650032,China)
出处
《中医药导报》
2018年第21期84-86,共3页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
军队医疗机构制剂标准提高计划专项课题重点项目(14ZJZ18):成都战区云南区域非标准制剂质量提升关键技术的系统研究
