摘要
本试验旨在探讨单胃动物仿生消化系统(SDS-2)消化条件及测定结果在不同实验室间的再现性。采用单因素完全随机设计,共4个实验室处理。将玉米、大豆粕粉碎过60目筛后,分发至4个实验室。每个实验室选用1台SDS-2,每台SDS-2设定2个仿生消化组测定其缓冲液流速、消化液流速、清洗液流速。通过温度传感器分别测定酶促反应室、缓冲液控温室、消化液储存室温度的变化曲线,通过电磁传感器测定混合频率的变化曲线。根据SDS-2操作技术规程,测定玉米及大豆粕的酶水解物能值。结果表明:1)不同实验室间小肠和大肠消化液流速有显著性差异(P<0.05),但其胃、小肠、大肠缓冲液流速及清洗液流速无显著性差异(P>0.05)。实验室内同一台SDS-2的2个仿生消化组间的胃、小肠、大肠缓冲液流速和清洗液流速均有显著性差异(P<0.05)。2)4个实验室中,虽然开机后仿生消化系统的起始混合频率以及酶促反应室、缓冲液控温室、消化液储存室的起始温度不同,但在运行60 min后消化条件均达到一致。3)玉米干物质消化率和酶水解物能值的实验室内、实验室间变异系数以及总变异系数均不超过1.23%,且实验室间无显著性差异(P>0.05)。大豆粕干物质消化率的实验室内、实验室间变异系数以及总变异系数分别为1.30%、2.23%和2.52%,酶水解物能值对应变异系数分别为0.89%、1.43%和1.64%,且实验室间有显著性差异(P<0.01)。由此可见,实验室间SDS-2小肠液、大肠液流速的差异并不导致饲料酶水解物能值测定结果的差异。各实验室SDS-2开机运行60 min以上可以达到一致的消化条件。实验室间玉米酶水解物能值测定的再现性高于大豆粕,2个饲料酶水解物能值的实验室总变异系数都可控制在1.64%以内,具有满意的再现性,达到定量分析要求。
This study was conducted to test the laboratory reproducibility of digestion condition and determination result in simulated digestion system （SDS-2） for monogastric animals. A single factor completely randomized design was adopted with 4 laboratory treatments. Corn and soybean meal were ground then passed a 60-mesh and delivered to the 4 laboratories. In each laboratory, one SDS-2 was selected to measure the flow velocities of buffer solution, digestive fluid and washing solution. Variation curves of the temperature in enzymatic reaction room, buffer solution controlled room and digestive fluid storage room were measured by temperature sensors, variation curves of the mixing frequency was measured by electromagnetic sensor. The enzymatic hydrolysate gross energy （EHGE） of corn and soybean meal was determined according to the manual of SDS-2. The results showed as follows： 1） there were significant differences in the flow velocity of digestive fluid in small intestine and large intestine among different laboratories （ P 〈0.05）, however, no significant differences were observed in the flow velocities of buffer solution in stomach, small intestine and large intestine and washing solution among different laboratories （ P 〉0.05）. There were significant differences in the flow velocities of buffer solution in stomach, small intestine and large intestine and washing solution in 2 simulated digestion groups in the same SDS-2 （ P 〈0.05）. 2） In the 4 laboratories, despite different initial mixing frequencies and initial temperatures in enzymatic reaction room, buffer solution controlled room, digestive fluid storage room, the consistent digestion condition was observed after the SDS-2 performed for 60 min. 3） In the corn, the intra-laboratory, inter-laboratory and total coefficient of variation （CV） were all less than 1.23% for dry matter digestibility （DMD） and EHGE, and no difference was observed across the laboratories （ P 〉0.05）. In the soybean meal, the intra-laboratory
作者
高庆涛
张虎
赵峰
王钰明
杜青之
邓耀辉
GAO Qingtao;ZHANG Hu;ZHAO Feng;WANG Yuming;DU Qingzhi;DENG Yaohui(State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Science,Beijing 100193,China;Hunan Zhongben Intelligent TechnologyDevelopment Co.,Ltd.,Changsha 410013,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2018年第9期3617-3625,共9页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家自然科学基金项目(31172215)
中国农业科学院青年英才计划
湖南中本智能科技发展有限公司横向合作项目(ZBZN20171127-01)
关键词
仿生消化
消化条件
酶水解物能值
再现性
simulated digestion
digestion condition
enzymatic hydrolysate gross energy
reproducibility
