摘要
具有高分辨率、能活体观察和三维成像的现代显微成像技术的产生和发展,为细胞生物学研究提供了有效的工具。我们在本文中阐述了几个重要的现代显微成像技术原理和技术要点以及在细胞生物学中应用的现状,包括光学显微术、电子显微术和荧光探针技术及相关技术,并对现代显微成像术的发展进行了展望。
Advanced microscopic imaging technology is a powerful tool in cell biology research. It can be used to achieve three-dimensional imaging in vivo with high resolution. This paper introduces the principles and key points of these techniques and the current situation of their applicaions in cell biology, including optical microscopy, electron microscopy, fluorescent probes and their related techniques. Moreover, the prospect of advanced microscopic imaging technology is also discussed.
作者
杨治河
闫丽
李红林
蔡亚非
YANG Zhi-he;YAN Li;LI Hong-lin;CAI Ya-fei(Department of Anatomy,ChuZhou City Vocation College,Anhui Chuzhou 239000,China;Department of Radiation Oncology Linyi People Hospital,Shandong Linyi 276003,China;Experiment Center,Shanghai lOth Hospital,Shanghai 200072,China;College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China)
出处
《解剖学报》
CAS
CSCD
北大核心
2018年第6期846-851,共6页
Acta Anatomica Sinica
基金
国家自然科学基金(31372207
81570094)
关键词
显微成像术
分辨率
活体观察
三维成像
人
Microscopic imaging technology
Resolution
Live observation
Three-dimensional imaging
Human
