摘要
【目的】本实验的目的是研究白背飞虱Sogatella furcifera控制不同眼色的基因以及眼色突变对交配能力的潜在影响。【方法】在实验室中构建了一种新型的白背飞虱橙色复眼突变体,与此性状相关的基因型符号为org。利用包含3种基因型(+/+,+/org,org/org)的8组杂交组合实验来确定影响橙眼性状的遗传基础,并通过一项交配竞争实验来测试复眼眼色突变是否对白背飞虱交配能力有影响。【结果】该种橙眼突变体具有一对与野生型白背飞虱有明显区别的亮橙色复眼。在突变体白背飞虱发育阶段均出现了橙眼性状:卵期眼点(产卵3 d后出现),若虫复眼和任意性别翅型的成虫复眼。纯合子正常眼白背飞虱和橙眼白背飞虱之间的互交产生了F1正常眼颜色的后代。卡方检验表明交配后代并未倾向于特定的性别(α=0. 05,χ2c=0. 19～1. 53)。橙眼白背飞虱突变体的交配竞争能力显著弱于野生型白背飞虱。【结论】橙眼性状由常染色体隐性基因控制,且控制眼色的org基因对交配竞争能力有显著影响。
【Aim】 This study aims to investigate the gene controlling the eye color of the white-backed planthopper （WBPH）, Sogatella furcifera and to explore the potential influence of eye color mutants on mating capacity. 【Methods】 An orange-eye mutant was established in our insectary, and the gene symbol related to this mutant was designated as org . The genetic basis of this phenotype was determined by designing eight groups of cross-mating experiment containing three genotypes （＋/＋, ＋/ org, org/org ）. Whether the eye color related genes influence the mating capacity in WBPH was tested in a mating competition experiment. 【Results】 This orange-eye mutant had a pair of bright orange ommatea which was distinctly different from the wild type WBPH. The orange-eye phenotype occurred in all developmental stages of WBPH： the eyespot of eggs （3 d after ovipisition） and the compound eyes of nymphs and adults of both sexes and wing morphs. Reciprocal crosses between homozygous normal-eye WBPH and orange-eye WBPH produced F 1 progeny with only normal-eye color. The sex ratio in the offspring produced from all groups was not biased to a specific sex based on Chi -square test （α=0.05, χ 2 c =0.19-1.53）. The mating competitiveness of the orange-eye mutant was significantly weaker than that of the wild type phenotype in WBPH. 【Conclusion】 Our results indicate that the inheritance of the orange-eye trait is controlled by an autosomal recessive allele, and eye color related gene has significant impacts on mating capacity in WBPH.
作者
葛家桢
楼玉婷
张传溪
GE Jia-Zhen;LOU Yu-Ting;ZHANG Chuan-Xi(Institute of Insect Sciences,Zhejiang University,Hangzhou 310058,China)
出处
《昆虫学报》
CAS
CSCD
北大核心
2018年第11期1356-1362,共7页
Acta Entomologica Sinica
基金
国家自然科学基金项目(31630057,31471765)
关键词
白背飞虱
眼色突变
橙眼
常染色体隐性基因
杂交试验
交配竞争
Sogatella furcifera
eye color mutant
orange-eye
autosomal recessive allele
hybridization experiment
mating competition
