摘要
目的利用动态增强MRI评价A2A腺苷受体激动剂瑞加德松对兔血－脑屏障的可逆性开放作用及最佳时间窗。方法将20只新西兰大白兔分为实验组和对照组，每组10只，分别经耳缘静脉注入瑞加德松和生理盐水，立即行脑冠状位扫描；随后注入Gd－二乙撑三胺五乙酸（DTPA）行同层间隙扫描（增强后5、10、15、20 min，之后每隔10 min），持续2 h，测量不同时间感兴趣区（ROI）的信号强度（SI），计算SI增强率（%）。观察兔脑伊文思蓝染色情况。采用配对t检验分析数据。结果实验组注入瑞加德松后5 min脑ROI的SI增强率为（40.93±3.70）%，明显升高，30 min左右达到最大值[（43.03±3.62）%]，持续至50 min缓慢下降，80 min左右趋于平稳；各时间点实验组SI增强率均高于对照组（t值：6.88～20.28，均P〈0.05），且实验组脑组织明显蓝染。结论瑞加德松可暂时可逆性开放血－脑屏障，最佳时间窗为注射后10～50 min。
ObjectiveTo evaluate the opening level and optimal time window of the blood-brain barrier induced by adenosine A2 receptor agonist （Lexiscan） via dynamic enhanced MRI.MethodsTwenty New Zealand white rabbits were divided into experiment group （group A, n=10） and control group （group B, n=10）. Rabbits in group A were injected with Lexiscan and rabbits in group B were injected with physiological salt via ear vein, then the coronary scanning was performed. Contrast enhanced MRI was performed at different time points （5, 10, 15, 20 min, and then every 10 min, until 2 h） following the infusion of Gd-diethylene triamine pentaacetic acid （DTPA）. The signal intensity （SI） of region of interest （ROI） was measured and the percent enhancement of SI was calculated. Evens blue staining results in brain tissues were observed. Pair t test was used to analyze the data.ResultsThe percent enhancement of SI in group A significantly increased to （40.93±3.70）% at 5 min, reached the maximum of （43.03±3.62）% at 30 min, slowly decreased until 50 min, and got to a stable level at almost 80 min. At each time point, the percent enhancement of SI in group A was significantly higher than that in group B （t values： 6.88-20.28, all P〈0.05）. The staining was evident in group A.
ConclusionsLexiscan can open blood-brain barrier temporarily and reversibly, and the optimal opening time window is 10-50 min post-injection.
作者
常灿灿
段阳
杨本强
周军
李虹易
徐志华
林森
Chang Cancan;Duan Yang;Yang Benqiang;Zhou Jun;Li Hongyi;Xu Zhihua;Lin Sen(Department of Radiology,the General Hospital of Shenyang Military Region,Shenyang 110016,China（Chang CC,Duan Y,Yang BQ,Li HY,Xu ZH,Lin S;Department of Radiology,the 4th People＇s Hospi-tal of Shenyang,Shenyang 110016,China（Zhou J)
出处
《中华核医学与分子影像杂志》
CAS
北大核心
2018年第11期741-744,共4页
Chinese Journal of Nuclear Medicine and Molecular Imaging
基金
辽宁省科技攻关基金（2012225019）
关键词
血脑屏障
腺苷A2受体激动剂
磁共振成像
钆DTPA
兔
Blood-brain barrier
Adenosine A2 receptor agonists
Magnetic resonance imaging
Gadolinium DTPA
Rabbits
