摘要
利用100 mg/L的茜素络合物(alizarin complexone,ALC)对鲤仔鱼进行48 h的水环境浸泡标记,以探讨该ALC标记方法的特征,及其对矢耳石、星耳石和微耳石的标记效果以及鱼体ALC浸泡、续养恢复与耳石ALC标记区域形成和消失的时滞进行研究。结果显示,3种耳石在可见光和荧光下均能检测到明显的标记环。其中星耳石的标记效果最佳,微耳石次之。耳石上荧光信号出现和消失的时间与鱼体ALC浸泡开始和结束的时间均存在1 d的时滞。此外,浸泡标记过的鲤仔鱼在进行了长达50 d的续养恢复后,其耳石上的ALC标记环仍清晰可见。研究表明,ALC标记法所形成的标记环在耳石上可长期存在,使用ALC对鲤仔鱼进行生态标记具有很强的可行性。
The artificial release is one of successful approaches for natural fishery stock enhancement. Therefore,effective marking techniques are expected to be applied for assessment of the contribution of released fishes to the fishery resources. Because fish at larval stages are better for batch marking and releasing, in the present study, larval common carp Cyprinus carpio were immersed in the 100 mg/L of alizarin complexone（ALC） for 48 h, so as to compare and investigate the marking characteristics of this method among sagitta, asteriscus and lapillus, as well as the possible time lag between start/end of fish ALC immersion and corresponding start/end of the fluorescent signal on otolith. Results suggested that marking rings in aforementioned three types of otolith were significantly observed in both visible and fluorescent lights. As for the ALC marking effect, asteriscus（the best） and lapillus（the second） were relatively better than sagitta. One-day time lag was found, respectively, between start of fish immersion and corresponding start of otolith marking, as well as end of fish immersion and corresponding end of ALC marking in extended area on otolith. The otolith ALC marking rings could still be obviously noted, even the fish was reared for 50 days after end of immersion. This phenomenon showed that ALC marking ring could be preserved on otolith for quite a long period. Consequently, the present method of ALC immersion will have strong feasibility for otolith ecological marking of larval C. carpio.
作者
邱晨
姜涛
陈修报
刘洪波
杨健
QIU Chen1,2, JIANG Tao2, CHEN Xiubao2, LIU Hongbo2, YANG Jian1,2(1. College of Wuxi Fisheries, Nanjing Agricultural University, Wuxi 214081, China; 2. Key Laboratory of Fishery Ecological Environment Assessment and Resource Conservation in Middle and Lower Reaches of the Yangtze River, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China)
出处
《水产学报》
CAS
CSCD
北大核心
2018年第11期1754-1765,共12页
Journal of Fisheries of China
基金
中央级公益性科研院所基本科研业务费专项(2016PT01)
农业财政专项(CJDC-2017-22)
关键词
鲤
耳石
茜素络合物
荧光标记
Cyprinus carpio
otolith
alizarin complexone （ALC）
fluorescence marking
