摘要
目的 探讨SLAMF6在重型再生障碍性贫血（SAA）CD8＋ T细胞中的表达情况及其与疾病免疫状态的相关性。方法 选取2017年2月至2018年4月天津医科大学总医院血液科收治的初治SAA患者21例，以15例健康人外周血标本作为正常对照，应用流式细胞术（FCM）检测外周血CD8＋ T细胞SLAMF6表达量，并与患者HGB、PLT、中性粒细胞绝对值、网织红细胞绝对值、骨髓造血功能（粒系百分比、红系百分比、淋系百分比、巨核细胞数）等临床指标及CD8＋ T细胞功能分子穿孔素、颗粒酶B、IFN-γ表达量进行相关性分析。进一步采用anti-SLAMF6 Ab阻断其功能，FCM检测CD8＋ T细胞穿孔素、颗粒酶B、IFN-γ分泌量。结果 初治SAA患者CD8＋ T细胞SLAMF6表达量明显低于正常对照，差异有统计学意义[（56.29±12.97）%对（80.96±7.36）%，t=-7.672，P〈0.001]；初治SAA患者CD8＋ T细胞SLAMF6表达量与HGB、PLT、中性粒细胞绝对值、网织红细胞绝对值、骨髓粒系百分比、骨髓红系百分比均呈正相关（P〈0.05），与CD8＋ T细胞穿孔素、颗粒酶B、IFN-γ表达量均呈负相关（P〈0.05）；anti-SLAMF6 Ab阻断该信号分子后，初治SAA患者CD8＋ T细胞anti-SLAMF6 Ab处理组穿孔素、颗粒酶B、IFN-γ表达量较未处理组明显增多，差异有统计学意义（P值均〈0.05）。结论 SLAMF6在SAA患者CD8＋ T细胞中明显低表达，其可能作为负性免疫调节分子通过影响CD8＋ T细胞功能分子的分泌参与SAA的发生机制。
Objective To explore the expression of SLAMF6 on CD8＋ T cells in patients with severe aplastic anemia （SAA） and its correlation with disease immune status.Methods By flow cytometry （FCM）, SLAMF6 expression level in peripheral blood CD8＋ T cells was detected in 21 patients with SAA and 15 normal controls respectively from February 2017 to April 2018. The correlation between SLAMF6 expression level and hematopoietic functions, including HGB, PLT, the neutrophil granulocyte and reticulocyte absolute value in peripheral blood, hyperplasia degree （percentage of granulocytes, erythrocytes, lymphocytes and megakaryocytes in bone marrow） and perforin, granzyme B, IFN-γ expression level in CD8＋ T cells were evaluated. To further confirm the effect of SLAMF6 on CD8＋ T cells, anti-SLAMF6 Ab was used to block SLAMF6 pathway （IgG as control）, and FCM was used to detect the perforin, granzyme B, and IFN-γ production of CD8＋ T cells.Results The expression of SLAMF6 on CD8＋ T cells in untreated SAA patients[（56.29±12.97）%]was significantly lower than that of normal controls[（80.96±7.36）%]（t=-7.672, P〈0.001）. The expression of SLAMF6 on CD8＋ T cells in SAA patients were positively correlated with the HGB, PLT, the neutrophil granulocyte and reticulocyte absolute value in peripheral blood, percentage of granulocytes, erythrocytes in bone marrow （all P〈0.05）, but they were negatively correlated with the percentage of lymphocytes in bone marrow, and the expression of perforin, granzyme B, and IFN-γ of CD8＋ T cells （all P〈0.05）. After blocking SLAMF6 pathway by anti-SLAMF6 Ab, the expression levels of perforin, granzyme B and IFN-γ in SAA patients were significantly higher than those in the untreated group, and the differences were statistically significant （all P〈0.05）.Conclusions SLAMF6 is significantly down-regulated on CD8＋ T cells in SAA patients, which may act as a negative immunoregulatory molecule participating in the mechanism of SAA by affecting the fun
作者
曾立洁
刘春燕
丁少雪
张田
邵宗鸿
付蓉
Zeng Lijie;Liu Chunyan;Ding Shaoxue;Zhang Tian;Shao Zonghong;Fu Rong(Department of Hematology,General Hospital,Tianjin Medical University,Tianjin 300052,China)
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2018年第11期927-931,共5页
Chinese Journal of Hematology
