摘要
目的:采用HPLC法测定大鼠血浆中替格瑞洛的浓度。方法:血浆样品采用甲醇沉淀蛋白,色谱柱为Inertsil ODS-3色谱柱(150 mm×4.6 mm,5μm),流动相为乙腈-10 mM乙酸铵水溶液(50∶50),流速为1.0 ml/min,紫外检测波长为299 nm。结果:大鼠血浆中替格瑞洛在0.1~5μg/ml范围内线性关系良好,标准曲线为A=32 465C+935.38,相关系数r=0.999 1。替格瑞洛的低、中、高浓度的提取回收率分别为85.21%、86.91%和85.87%;日内、日间精密度RSD均小于10%。结论:本法简便、快速、可靠,适用于检测血浆中替格瑞洛的浓度。
Objective：To establish an HPLC method for determination of Ticagrelor in rat plasma.Method：The rat plasma samples were prepared by protein precipitation with methanol.HPLC analysis of plasma samples was performed with a Inertsil ODS-3（150 mm×4.6 mm,5 μm）column.The mobile phase consisted of a mixture of 10 mM ammonium acetate buffer and acetonitrile（50∶50,v/v）,at a flow rate of 1.0 ml/min.The wavelength of 299 nm was used for detection.Result：A good linearity of ticagrelor in the rat plasma was shown in the range of 0.1-5 μg/ml（r=0.999 1）.The regression equation was A= 32 465C＋935.38.The extraction recoveries of ticagrelor in low,middle and high concentrations were 85.21%,86.91% and 85.87%,respectively.The intra-day and inter-day variation（RSD） were both less than 10%.Conclusion：The method is simple,fast,and reliable,and it can be employed to determine the concentration of Ticagrelor in plasma.
作者
马文娟
MA Wenjuan(First Affiliated Hospital of Fujian Medical University,Fuzhou 350005,China)
出处
《中外医学研究》
2018年第32期186-188,共3页
CHINESE AND FOREIGN MEDICAL RESEARCH
