摘要
为研究运动后不同休息时间对间歇性低氧诱发心肌自噬反应的影响，本研究将8周龄Sprague-Dawley雄性大鼠，随机分成控制组（control group，CON）、运动组（exercise group，EXE）、间歇性低氧曝露组（intermittent hypoxia group，IH）、空气曝露组（room air group，RA）、运动后1h（post-exercise 1 h，PE1h）和3h（PE3h）合并RA或IH组。大鼠给予单次持续8hIH（氧气浓度下降至2％～6％约1～2s／75s），单次运动方式以速度24m／min、坡度2％的强度在动物跑台上跑步60min。动物牺牲后，检测左心室心肌糖原、自噬反应相关蛋白及腺粒体功能相关mRNA表达量。研究发现，运动后心肌细胞微管相关蛋白1轻链3-Ⅱ（microtubule-associated protein 1 light chain 3-Ⅱ，LC3-Ⅱ）／LC3-Ⅰ表达量、糖原含量下降（p〈0．05），p62表达量没有变化（p〉0.05）。IH对心肌p62表达量没有影响（p〉0.05），与RA比较，IH的LC3-Ⅱ／LC3-Ⅰ、核内呼吸因子1和2（nuclear respiratory factor 1 and 2，NRF-1和NRF-2）mRNA表达量上升（p〈0．05）。与IH比较，PE1h＋RA、PE3h＋RA及PE3h＋IH各组LC3-Ⅱ／LC3-Ⅰ、NRF-1和NRF-2mRNA表达量均下降（p〈0．05），虽然PE1h＋IH的NRF-1和NRF-2 mRNA表达量下降（p〉0．05），但LC3-Ⅱ／LC3-Ⅰ表达量没有改变（p〉0．05）。本研究说明，IH曝露前3h进行运动，可避免IH造成心肌LC3-Ⅱ／LC3-Ⅰ表达量上升的现象。
To investigate the effects of different time intervals after exercise on intermittent hypoxia （IH）-induced autophagy in rat left ventricle, eight-week-old male Sprague-Dawley rats were randomly assigned to control （CON）, exercise （EXE）, IH, room air exposure （RA）, post-exercise 1 h and 3 h combined with RA （PElh ＋RA and PE3h＋RA） orIH （PElh＋IHand PE3h＋IH） groups. Rats were exposed to a single IHbout （2%-6% 02 for 1-2 s/75 s） for 8 h and a single exercise bout on treadmill （24 m/min, 2% grade） for 60 min. Myocardial glycogen, autophagy- related proteins and mitochondrial-related mRNAs were determined after euthanisation. It could be found that immediately after exercise, microtubule-associated protein 1 light chain 3- Ⅱ （LC3-Ⅰ/）/LC3-Ⅰ protein levels decreased （/9〈0.05） but p62 levels did not alter （p〉0.05）. As compared with RA, IH did not alter p62 levels but did increase LC3- U/LC3-Ⅰ protein and nuclear respiratory factor 1 and 2 （NRF-1 and NRF-2） mRNA levels （p〈0.05）. Compared with IH, significantly lower LC3-Ⅱ/LC3-Ⅰ protein and NRF- 1 and NRF2 mRNA levels were found in PElh＋RA, PE3h＋RA and PE3h＋IH （p〈0.05）. NRF-1 and NRF-2 mRNA levels were lower in PE 1 h＋IH than inlH （p〈0.05）, although LC- Ⅱ/LC3-Ⅰ levels were similar （p〉0.05）. It could be concluded that exercise at 3 h before IH exposure could prevent IH-induced increased myocardial LC3-Ⅱ/LC3-Ⅰ levels.
作者
田应娟
Tian Yingiuan(College of Physical Education,Kunming College,Kunming,650214)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2018年第11期5077-5085,共9页
Genomics and Applied Biology
基金
昆明学院体育学院资助
关键词
心脏
氧化压力
心肌自噬
低氧
Heart
Oxidative stress
Myocardial cells autophagy
Hypoxia
