摘要
目的检测白色念珠菌在新疆博尔塔拉蒙古自治州蒙古族、维吾尔族及汉族儿童口腔中的分布，并探讨其与低龄儿童龋的相关性，以期为龋病的早预测及早预防提供依据。方法采用多阶段、分层、整群抽样、随机数字表法，对新疆博尔塔拉蒙古自治州1 089名3-5岁健康儿童（蒙古族136名、维吾尔族403名、汉族550名；男性568名、女性521名）进行横断面研究，按照标准分为低龄儿童龋（early childhood caries，ECC）组786名，无龋组303名。采集有龋组龋坏组织样本（取所有龋坏牙表面的牙体组织）和无龋组龈上菌斑，利用鉴别培养基分离培养，并通过革兰染色、芽管试验、PCR及内转录间隔区测序进行鉴定，PCR-25SrDNA法进行基因分型。对不同基因型白色念珠菌在不同民族、不同性别间的差异和龋失补牙数分级与白色念珠检出的关系运用Pearson χ2检验和Spearman相关分析进行统计学分析。结果样本检测结果显示，总样本中，ECC组白色念珠菌检出率[14.4%（113/786）]显著高于无龋组[7.6%（23/303）]（χ2=9.214，P=0.002）；维吾尔族白色念珠菌检出率[17.9%（72/403）]显著高于汉族[8.7%（48/550）]（χ2=17.648，P=0.000），三民族检出率差异有统计学意义（χ2=17.848，P=0.000）。男性儿童中，维吾尔族白色念珠菌检出率[21.7%（44/203）]显著高于汉族[8.4%（24/285）]（χ2=17.365，P=0.000），三民族检出率差异有统计学意义（χ2=18.887，P=0.000）；女性儿童中，蒙古族[14.3%（8/56）]、维吾尔族[14.0%（28/200）]及汉族白色念珠菌检出率[9.1%（24/265）]差异无统计学意义（χ2=3.206，P=0.201）。ECC组维吾尔族和汉族口腔白色念珠菌检出率与龋失补牙数分级具有显著相关性（维吾尔族：r=0.195，P=0.001；汉族：r=0.145，P=0.004）。136例白色念珠菌样本中PCR-25SrDNA分3型，以A型为主[55.1%（75/136）]。结论低龄儿童口腔
Objective To investigate the distributions of Candida albicans （Ca）, which are closely associated to early childhood caries （ECC） of young children, in 3-5-year-old children of Mongolian, Uygur and Han ethnic groups in Bortala Mongol Autonomous Prefecture of Xinjiang Urgur Autonomous Region, so as to provide scientific basis for the early prediction and prevention of caries locally.Methods Totally 1 089 healthy children aged 3 to 5 years old （Mongolian 136, Uygur 403 and Han 550; 568 males and 521 females） were randomly selected by stratified and cluster sampling method. The children in early childhood caries group were 786 and in caries free group were 303. Dental plaque samples were collected from the carious tooth tissues and supragingival sites, respectively. Samples were isolated and cultured by different media. Ca were identified by Gram stain, germ tube test, PCR method and internal transcribed spacer region sequencing methods. Ca isolated were further typed by using PCR-25SrDNA genotype method. Pearson χ2 test and Spearman rank correlation were used to analyze the differences in genotypes of Ca amongst three ethnic groups and the relationship between decayed missing filled tooth （dmft） classification and detection of Ca.Results The Ca was significantly higher in the ECC group [14.4% （113/786）] than in the caries free group [7.6% （23/303）] （P=0.002）. The identified rate of Ca in Mongolian group was [11.8% （16/136）], which was significantly lower than that of Uygur group [17.9% （72/403）] and higher than that of Han group [8.7% （48/550）] （χ2=10.192, P=0.006）. Among the male children, the identified rate of Ca in Mongolian group was [10.0% （8/80）], which was significantly lower than that of Uygur group [21.7% （44/203）] and higher than that of Han group [8.4% （24/285）] （χ2=18.887, P=0.000）. Among the female children, the detection rates of Ca were [14.3% （8/56）] in Mongolian group, [14.0% （28/200）] in Uygur group and [9.1% （24/265）] in
作者
曹宏飞
董英
杨婷
李贝贝
赵今
Cao Hongfei;Dong Ying;Yang Ting;Li Beibei;Zhao Jin(Department of Endodontics,The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China)
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2018年第11期730-735,共6页
Chinese Journal of Stomatology
基金
国家自然科学基金（81760194）
关键词
念珠菌
白色
龋齿
基因型
低龄儿童龋病
Candida albicans
Dental caries
Genotype
Early childhood caries
