摘要
以洮南绿豆种子为试材,采用不同浓度硝酸亚汞、硝酸铅和氯化铜溶液单一处理绿豆种子,探究了重金属离子胁迫对绿豆萌发过程中生理指标、可溶性糖和可溶性蛋白质含量的影响,以期为进一步了解重金属对农作物萌发和生长的毒害机制提供参考依据。结果表明:3种重金属离子溶液胁迫均能抑制绿豆幼苗根和茎的生长,其中氯化铜、硝酸亚汞胁迫的影响较为显著。另外,绿豆种子在经重金属离子溶液胁迫后,幼苗全株的可溶性糖含量明显增加。其中当硝酸亚汞和硝酸铅浓度分别达到50mg·L-1和150mg·L-1时,绿豆幼苗的可溶性糖含量达到最大值。此外,硝酸铅胁迫促进绿豆幼苗内可溶性蛋白质的积累,而硝酸亚汞和氯化铜胁迫对可溶性蛋白质含量没有明显影响。
In order to explore the toxic mechanism of heavy metals stress on the germination and growth of crops,the green bean seeds were used as materials,the seed germination and seedling physiological indices of green bean were compared and analyzed under different concentrations of Hg2（NO3）2,Pb（NO3）2 and CuCl2 treatment.The results showed that the stress of three heavy metal ions could inhibit the growth of root and stem of green bean seedlings,especially the CuCl2 and Hg2（NO3）2 stress.However,the soluble sugar content of green bean seeds increased significantly under the heavy metal ions stress.Under the stress of 50 mg·L^-1 Hg2（NO3）2 and 150 mg·L^-1 Pb（NO3）2,the accumulation of soluble sugar got to the peak.Moreover,Pb（NO3）2 stress promoted the accumulation of soluble protein in germinated green bean,rather than Hg2（NO3）2 and CuCl2 stress.
作者
齐文靖
于晗
张佳慧
张萌祺
徐盛世
崔月
QI Wenjing;YU Han;ZHANG Jiahui;ZHANG Mengqi;XU Shengshi;CUI Yue(Department of Bioscience,Changchun Normal University,Changchun,Jilin 130032)
出处
《北方园艺》
CAS
北大核心
2018年第21期1-5,共5页
Northern Horticulture
基金
吉林省教育厅"十三五"科学技术资助项目(JJKH20170656KJ)
长春师范大学自然科学基金资助项目(2015-001)
2017年吉林省大学生创新创业训练计划资助项目(201710205074)
关键词
重金属
种子萌发
可溶性糖
可溶性蛋白质
绿豆
heavy metal ions
seed germination
soluble sugar
soluble protein
green bean
