摘要
为研究日本蛇根草功能基因的表达提供内参基因,笔者参照GenBank中桔梗的肌动蛋白基因(Actin)序列设计引物,以日本蛇根草的cDNA为模板,通过RT-PCR方法成功克隆获得日本蛇根草Actin基因片段,并将其命名为OjActin(OjActin片段长469 bp)。功能保守域分析显示OjActin归属于Actin超家族,与其他植物Actin的氨基酸序列同源性达94%以上;系统进化树分析显示,OjActin与咖啡的亲缘关系最近。
In order to provide control gene for the study of functional genes expression of Ophiorrhiza japonica , we designed the primers on the basis of Aticn from Eustoma in the Genebank. We successfully cloned the Actin gene fragment by RT-PCR method using the cDNA of Ophiorrhiza japonica as template, and named it as OjActin（ OjActin was 469 bp and belonged to the Actin superfamily）. OjActin shared over 94% amino acid sequence homology with Actins from other plants, and had the nearest relationship with Actin from Coffea canephora according to polygenetic analysis.
作者
孙威
徐僡
林建
马玲
申欢
李欲轲
乙引
SUN Wei;XU Hui;LIN Jian;MA Ling;SHEN Huan;LI Yuke;YI Yin(Key Laboratory of Plant Physiology and Development Regulation,School of Life Sciences,Guizhou Normal University,Guiyang,Guizhou 550025,China;Key Laboratory of State Forestry Administration on Biodiversity Conservation inKarst Mountain Area of Southwest of China,Guizhou Normal University,Guiyang,Guizhou 550025,China)
出处
《贵州师范大学学报（自然科学版）》
CAS
2018年第6期38-42,67,共6页
Journal of Guizhou Normal University：Natural Sciences
基金
国家自然科学基金(31760076)
贵州省教育厅青年科技人才成长项目(黔教合KY字[2017]122)
贵州省联合基金项目(黔科合LH字[2016]7211号
黔科合LH字[2017]7358)
贵州师范大学资助博士科研项目(0516006)
贵州省重点实验室建设项目(黔科合计Z字[2011]4005)
