摘要
目的探究miR-183在急性心肌梗死(AMI)患者和健康志愿者血浆中的表达,分析AMI患者与健康志愿者间的临床病理学参数的差异,分析miR-183表达水平对AMI患者预后的影响。方法选取该院2010年8月至2015年8月期间收治的经确诊为AMI的患者33例为AMI组,另选取健康志愿者33例为对照组,收集其临床病理学资料,采用实时荧光定量PCR(qPCR)检测miR-183在2组血浆中的表达。分析2组临床病理学参数的差异以及差异参数与miR-183表达水平之间的相关性,分析miR-183的表达水平与总生存期(OS)和无病生存期(DFS)之间的关系。结果 miR-183在AMI组患者血浆中的表达水平显著高于对照组,差异有统计学意义(P<0.05);2组受试者的年龄、性别、吸烟史、载脂蛋白B、高密度脂蛋白(HDL)和低密度脂蛋白(LDL)比较差异均无统计学意义(P>0.05),而载脂蛋白A1、白细胞、肌酸肌酶同工酶、心肌肌钙蛋白T和射血分数比较差异有统计学意义(P<0.05),且AMI组患者中miR-183的表达水平与白细胞、肌酸肌酶同工酶、心肌肌钙蛋白T呈正相关(P<0.05),与射血分数和载脂蛋白A1呈负相关(P<0.05);miR-183高表达的AMI患者的OS和DFS显著低于低表达患者,差异有统计学意义(P<0.05)。结论 miR-183在AMI患者血浆中呈高表达,且AMI患者的部分临床病理学参数也发生显著变化;miR-183与AMI患者预后相关,有可能作为AMI患者预后判断的特异性分子标记物。
Objective To explore the expression of circulating miR 183 in the plasma of acute myocardial infarction （AMI） patients and healthy volunteers,and to analyze the difference of clinical parameters between AMI patients and healthy volunteers and the significance of miR 183 expression in predicting the prognosis of AMI patients. Methods 33 patients who were diagnosed with AMI in the hospital from August 2010 to Au gust 2015 were selected as the AMI group,and other 33 cases of healthy volunteers were selected as control group. The clinical pathological data of two groups were collected. The quantitative real time PCR （qPCR） was used to detect the expression of miR -183 in the plasma of patients between the two groups. The difference of clinicopathological parameters between the two groups and its correlation with the expression of miR -183 were analyzed. The correlation between miR- 183 expression and overall survival （OS） and disease free survival （DFS） were analyzed. Results The expression of plasma miR 183 in the AMI group was significantly higher than that in the control group, and the difference was statistically significant （P〈0.05）. There were no signif icant differences between the two groups in age,gender, smoking history,apolipoprotein B, high density lipo protein （HDL） and low density lipoprotein （LDL） （P〉0. 05） ,while there were significant differences in the apolipoprotein Al,white blood cells,creatine kinase MB,cardiac troponin T and ejection fraction （P〈0.05）. The expression of miR- 183 in the AMI group was positively correlated with white blood cells,creatine kinase MB and cardiac troponin T, and negatively correlated with ejection fraction and apolipoprotein A1, and the difference was statistically significant （P〈0.05）. OS and DFS in the AMI group with high expression miR- 183 were significantly lower than those with low expression miR- 183, and the difference was statistically significant （P〈0. 05）. Conclusion pathological parameters of AMI and ma
作者
张艳
翁方中
徐珍
孙云飞
彭勇
ZHANG Yan;WENG Fangzhong;XU Zhen;SUN Yunfei;PENG Yong(Department of ICU,the Eleventh Hospital of Wuhan,Wuhan,Hubei 430015,China)
出处
《国际检验医学杂志》
CAS
2018年第22期2809-2812,2816,共5页
International Journal of Laboratory Medicine
