摘要
外切酶Ⅲ(Exonuclease Ⅲ,Exo Ⅲ)是一种可以特异性识别并催化dsDNA从3'-羟基端逐步水解并释放出单核苷酸的核酸酶,而对ssDNA的水解作用十分有限。基于Exo Ⅲ的外切酶活性,搭载不同的信号输出方式,该酶已被成功运用到DNA、小分子和金属离子等靶物质的放大检测中。本文主要根据所检测的靶物质不同,对Exo Ⅲ介导的生物传感器进行了分类综述,阐述了各传感器的基本设计原理和灵敏度等方面内容。此外,对Exo Ⅲ与其他信号放大策略或纳米材料相结合的最新研究进展也做了较为详细的介绍。最后,对Exo Ⅲ介导的生物传感器的优缺点和发展前景进行了总结和展望,有助于Exo Ⅲ介导的生物传感器在环境监管、生物分析或临床诊断领域的深层次应用和继续研发。
Exonuclease Ⅲ（Exo Ⅲ）is a kind of nuclease that can specifically recognize dsDNA and catalyze the release of mononucleotides by stepwise hydrolysis from 3＇-hydroxyl termini of dsDNA strands;while hydrolysis on ssDNA is limited. Based on itsexonuclease activity,Exo Ⅲ has been successfully employed for amplified detection of DNAs,small molecules and metal ions while combinedwith a series of signal outputs. Here we mainly give a detailed classifying description of the Exo Ⅲ-assisted biosensors based on various targetsubstances,and also expound the working principles and sensitivity of each sensor. In addition,we discuss the recent progresses on theintegration of Exo Ⅲ-assisted target-recycling strategy with other signal enhancement strategies or emerging nanomaterials in detail as well.Finally,we summarize the advantages and disadvantages of the Exo Ⅲ-assisted biosensors and prospect the future development,which will beconducive to further application and continuous development of Exo Ⅲ-assisted biosensors in environmental management,biological analysis and clinical diagnosis.
作者
宋欢
罗云波
许文涛
SONG Huan;LUO Yun-bo;XU Wen-tao(Beijing Advanced Innovation Center for Food Nutrition and Human Health,College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083;Key Laboratory of Safety Assessment of Genetically Modred Organism（Food Safety）,Ministry of Agriculture,Beijing 100083)
出处
《生物技术通报》
CAS
CSCD
北大核心
2018年第9期55-69,共15页
Biotechnology Bulletin
基金
国家自然科学基金项目(31671922)
北京市科技新星计划(xxjc 201721)
关键词
外切酶Ⅲ
功能核酸
信号放大
生物传感器
exonuclease Ⅲ
functional nucleic acid
signal amplification
biosensor
