摘要
目的 :探讨复发难治性急性白血病多药耐药性及耐药性的逆转。方法 :采用RT PCR法检测白血病细胞的多药耐药性 ,采用MTT法进行体外药敏及耐药逆转试验。结果 :35例患者中MDR1mRNA阳性表达例数为 2 6例(74.3% ) ,体外耐药逆转环胞素组逆转率 6 4.7%、异搏定组 41.2 %、两药合用组 76 .5 % ;临床耐药逆转环胞素组逆转率 14.3%、异搏定组 45 .8%、两药合用 37.5 % ;结论 :复发难治性急性白血病MDR1mRAN高表达 ,环胞素和异搏定单用及两药联合应用体外逆转效果明显优于临床疗效 。
Objective:To evaluate expression of the mdr1 gene and reversal of drug resistance on refractory-relapsed acute leukemia.Methods:The expression of multidrug resistance gene (mdr1 mRNA) was measured by reverse transcription polymerase chain reaction (RT-PCR) and chemosensitivity and reversal of drug risistance assay in vitro was measured by MTT methods.Results:The mdr1 positive rate in refractory-relapsed acute leukemia (AL) was 74.3%.Reversal rate in vitro in groups CSA?VPL?CSA plus VPL were 64.7%?41.2%?76.5% respectively and reversal rate in clinic were respectively 14.3%?45.8%?37.5%.Conclusion: The mdr1 mRNA was over expressed in refractory-relapsed AL.Reversal rate in vitro was higher than that in clinic with drug resistance modifiers (CSA VPL CSA and VPL).Dosage of drug resistance modifier and way of giving drug may be important factors affecting effect.
出处
《实用临床医学（江西）》
CAS
2002年第A03期51-53,共3页
Practical Clinical Medicine
关键词
白血病
复发难治
多药耐药
逆转
Luekemia
refractory-relapsed
multidrug resistance
reversal
