摘要
以长穗偃麦草(Elytrigia elongata)幼穗为外植体, MS为基本培养基,研究了不同植物生长调节物质配比对其愈伤组织诱导、分化和生根的影响。结果表明,长穗偃麦草幼穗诱导最佳培养基为MC+3 mg·L-1 2,4-D,诱导率达66.67%, 4周后可见淡黄色愈伤组织。最佳分化培养基为MC+0.1 mg·L-1 2,4-D+3mg·L-1 6-BA,分化率为64.44%, 4周后出现芽点,同时伴随根毛发生。最佳生根培养基为MR+0.5 mg·L-1 NAA,生根率为100%,移栽后全部成活?
The tissue culture and rapid propagation system of Elytrigia elongata was established by using the panicle as explants and MS as the basic callus. The effects of various plant grouth regulators combinations on callus induction, differentiation and rooting were studied. The results showed that the optimal medium for induction of panicles of E. elongata was MC＋3 mg·L^-1 2,4-D, and the induction rate was 66.67%. After 4 weeks, light yellow callus was observed. The optimal differentiation medium was MC＋0.1 mg·L^-1 2,4-D＋3 mg·L^-16-BA, and the differentiation rate was 64.44%. After 4 weeks, the bud point appeared, accompanied by root hair. The best rooting medium was MR＋0.5 mg·L^-1 NAA, the rooting rate was 100%, and all survived after transplanting.
作者
周妍彤
张琳
郭强
田小霞
孟林
崔国文
ZHOU Yan-Tong;ZHANG Lin;GUO Qiang;TIAN Xiao-Xia;MENG Lin;CUI Guo-Wen(College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China;Beijing Research and Development Center for Grass and Environment,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2018年第9期1475-1480,共6页
Plant Physiology Journal
基金
北京市自然科学基金(6182013)
北京市农林科学院科技创新能力建设专项(KJCX20170110)~~
关键词
长穗偃麦草
幼穗
组织培养
植株再生
Elytrigia elongata
panicle
tissue culture
plant regeneration
