摘要
研究高粱原花青素(sorghum procyanidins,SPC)对生长育肥猪生长性能、养分消化率、消化酶活性、免疫力和抗氧化能力的影响。160头70日龄、平均体重为(31. 28±1. 12) kg的杜×长×大三元杂交猪,按照单因素完全随机设计分为4个组,分别饲喂玉米-豆粕基础日粮,以及基础日粮中添加100、150、200 mg/kg SPC。实验期为14周,生长期和育肥期各7周。在每个实验阶段开始和结束时称重,每天记录采食量,计算各阶段平均日增重(ADG)、平均日采食量(ADFI)和料重比(F/G)。在每个实验阶段的最后3 d,收集粪样测养分消化率。在每个实验阶段结束时,采血测血清抗氧化指数和免疫参数,同时,每个重复随机屠宰2头猪,取胰腺组织,测消化酶活性。结果表明,日粮添加SPC对生长育肥猪的生长性能没有显著的促进作用(P> 0. 05)。在生长猪阶段,日粮添加150 mg/kg和200 mg/kg SPC显著降低了消化酶的活性,对养分消化率有降低的趋势。在育肥猪阶段,日粮添加150 mg/kg显著提高了粗蛋白的表观消化率(P <0. 05),200 mg/kg SPC显著地抑制了消化酶的活性(P <0. 05)。与对照组相比,不论是在生长期还是育肥期,添加150 mg/kg或200 mg/kg的SPC均显著地增强了猪血清谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)的活性以及血清总抗氧化能力(T-AOC)(P <0. 05),降低了血清丙二醛(MDA)浓度(P <0. 05),提高了猪血清IgG、IgM和白细胞介素2(IL-2)的浓度(P <0. 05)。总之,SPC有利于提高生长育肥猪抗氧化能力,增强免疫力,但是对于生长育肥猪的生长性能及养分消化率没有显著的促进作用,相反,高浓度的SPC甚至抑制了消化酶的活性,从而影响养分的消化利用。
The trial was conducted to investigate effects of sorghum procyanidins （SPC） on growth performance,nutrient digestibility,digestive enzyme activities,immunity and antioxidant capacity in growing-finishing pigs. A total of 160 Duroc × Landrace × Yorkshine of 70 d of age （average weight（31. 28 ± 1. 12） kg） were randomly assigned to four treatment groups and fed with a basal corn-soybean meal diet （BD）,and BD ＋ 100,150,200 mg/kg SPC respectively. The trial lasted for 14 weeks. The growing period and the fattening period were seven weeks respectively. The body weight of pigs was recorded at the beginning and end of each trial phase,and the feed intake was recorded daily. The average daily gain （ADG）,average daily feed intake （ADFI） and feed/gain ratio（F/G） in each period were calculated. Feces were collected for last 3 days at the end of each trial phase to determine the nutrient digestibility. Blood samples were collected at the end of each trail phase for the measurement of serum immune parameters and antioxidant indices. Two pigs were randomly slaughtered and the pancreatic tissue was collected for measurement of digestive enzyme activities. The results indicated that dietary SPC supplementation did not exert significant promotion effects on growth performance in growing-finishing pigs （P〉0. 05）. At the growing period,the activities of digestive enzymes reduced significantly and the nutrient digestibility showed a downward trend due to the supplementation of SPC at concentrations of 150 and 200 mg/kg. At the fattening period,SPC supplementation at concentration of 150 mg/kg enhanced the apparent digestibility of crude protein observably （P〈0. 05）,whereas SPC supplementation at concentration of 200 mg/kg inhibited the activities of digestive enzymes significantly（P〈0. 05）. Either at the growing period or fattening period,pigs offered diets supplemented with SPC at dose of150 or 200 mg/kg produced stronger （P〈0. 05） serum total antioxidant capacity （T-AOC）
作者
闫慧诗
高俊杰
高文伟
李清宏
郝瑞荣
Yan Huishi;Gao Junjie;Gao Wenwei;Li Qinghong;Hao Ruirong(College of Animal Science and Veterinary Medicine,Shanxi Agricuhural University,Taigu 030801;Shanxi Agricultural University Library,Taigu 03080;Shanxi Collaborative Innovation Center for High-Productive and Safe Livestock,Taigu 030801)
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2018年第10期84-90,97,共8页
Journal of the Chinese Cereals and Oils Association
基金
山西省科技攻关项目(20120311022-2)
山西省重点研发项目(201603D221027-2)
关键词
高粱原花青素
生长育肥猪
消化率
消化酶活性
免疫力
抗氧化能力
sorghum procyanidins （SPC）
growing -finishing pigs
digestibility
digestive enzyme activities
immunity
antioxidant capacity
