摘要
设计了一种基于催化发夹结构自组装(CHA)技术的电化学传感器,用于miRNA21的检测。miRNA21启动CHA循环体系,最终产生大量的发夹H1-H2杂化双链,双链DNA产物与电极表面的捕获探针杂交,生物素标记的双链DNA和链霉亲和素标记的碱性磷酸酶(ST-AP)进一步结合,通过酶催化反应放大电化学信号。实验条件优化后,该传感器在1～1×105pmol/L浓度范围内具有良好的线性关系,其检测限为0. 92 pmol/L。
In this paper, a simple electrochemical biosensor was developed for detection of miRNA21 using catalytic hairpin assembly （CHA）. The miRNA21 could initiate the CHA cycles, resulting in the generation of massive hairpin H1 - H2 complexes. As a result, the generated dsDNA complexes were hybridized with capture probe attached on the sensor surface. Streptavidin-alkaline phosphatase （ST-AP） was then bound to biotinylated dsDNA, leading to enzymatic signal amplification.. Under optimized conditions, this newly designed biosensor showed good linearity in the concentration of 1 - 1 × 10 5 pmol/L with a detection limit of 0.92 pmol/L. The developed biosensor exhibited excellent sensitivity and selectivily.
作者
王鹏
李青
WANG Peng ,LI Qing(Department of Clinical Laboratory, Xuzhou Central Hospital Xuzhou 221009)
出处
《分析试验室》
CAS
CSCD
北大核心
2018年第11期1327-1331,共5页
Chinese Journal of Analysis Laboratory
