摘要
目的对老挝占巴塞省按蚊种类及其疟原虫子孢子感染情况进行调查,为当地疟疾防治措施的制定与评估提供参考。方法2017年7月在老挝占巴塞省巴通坡县采用诱蚊灯通宵诱蚊法和通宵人诱法进行捕蚊,对捕获的成蚊进行形态学鉴定,取部分雌性按蚊提取基因组DNA,巢式PCR检测恶性疟原虫和间日疟原虫的18S rRNA基因,计算按蚊疟原虫子孢子阳性率。结果共捕获蚊虫34 687只,分属库蚊、按蚊和伊蚊等8个属的29个种。库蚊属为当地优势属,占捕获蚊虫总数的92.6%(32 110/34 687);其次是按蚊属,占5.6%(1 947/34 687)。迷糊按蚊是按蚊属的优势种,占该属的65.5%(1 275/1 947);其后是可赫按蚊,占12.1%(235/1 947);菲律宾按蚊,占11.9%(232/1 947);中华按蚊,占5.1%(100/1 947)。巢式PCR共检测按蚊336只(可赫按蚊234只、中华按蚊100只和大劣按蚊2只), 8只按蚊间日疟原虫子孢子阳性,阳性率为2.4%(8/336)。其中,可赫按蚊子孢子阳性6只,阳性率为2.6%(6/234);中华按蚊子孢子阳性2只,阳性率为2.0%(2/100);均未检测到恶性疟原虫子孢子。巢式PCR扩增片段长120 bp,其序列与间日疟原虫序列(GenBank登录号为:KT991325、 KT991317、 MG708221、 MF540772、 LT635613、 KU569498、 AF145335、 KT991314和KX007932)的同源性为99%～100%。结论老挝占巴塞省捕获的可赫按蚊和中华按蚊存在间日疟原虫子孢子自然感染,且阳性率均较高。
Objective To investigate the species of Anopheles and rate of Plasmodium sporozoites in anopheline mosquitoes in Champasak Province of Laos, so as to provide essential data for malaria control.Methods Mosquitoes were captured by overnight trapping with light traps and by outdoor human landing catches in Batong County of Champasak Province in July 2017.After morphological identification, genomic DNA was extracted from some female adult mosquitoes, and 18S rRNA-based nested-PCR was performed to detect soprozoites of Plasmodium falciparum and P.vivax.The sporozoite positive rate of Anepheles was calculated.Results A total of 34 687 mosquitoes were captured, belonging to 29 species of 8 genera, dominated by the genus Culex（92.6%, 32 110/34 687）,followed by genus Anopheles（5.6%, 1 947/34 687）.The genus Anopheles was dominated by An.vagus（65.5%,1 275/1 947）, followed by An.kochi（12.1%, 235/1 947）, An.philippinensis（11.9%, 232/1 947）, An.sinensis（5.1%, 100/1 947）.A total of 336 anopheline female mosquitoes（comprising 234 An.kochi, 100 An.sinensis and2 An.dirus） were examined by nested-PCR, of which 8 were detected to be positive for P.vivax, including 6 An.kochi（positive rate 2.6%, 6/234） and 2 An.sinensis（2.0%, 2/100）.No soprozoites of P.falciparum were found.Nested-PCR amplification showed a band of ～120 bp, sequence of which had 99%-100% homology to those of P.vivax（GenBank Accession No： KT991325, KT991317, MG708221, MF540772, LT635613, KU569498, AF145335,KT991314 and KX007932）.Conclusion Both An.kochi and An.sinensis mosquitoes carry P.vivax sporozoites with a positive rate of 2.6% and 2.0% respectively.
作者
王微莉
杨锐
罗春海
郭晓芳
姜进勇
周红宁
Somphad SORCHAMPA
Phoxay VANNAPASEUTH
张苍林
WANG Wei-li;YANG Rui;LUO Chun-hai;GUO Xiao-fang;JIANG Jin-yong;ZHOU Hong-ning;Somphad SORCHAMPA;Phoxay VANNAPASEUTH;ZHANG Cang-lin(School of Public Health,Kunming Medical University,Kunming 650500,China;Yunnan Provincial Key Laboratory of Vector-borne Diseases Control and Research,Yunnan Provincial Center of Malaria Research,JIN Ning-yi Academic Station,Yunnan Institute of Parasitic Diseases,Pu＇er 665099,China;Sing District Hospital of Louang Namtha Province,Mengxin County 01000,Laos;Malaria and Parasitology Station of Champasak Province,Pakse County 01000,Laos)
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2018年第5期478-482,共5页
Chinese Journal of Parasitology and Parasitic Diseases
基金
中国-大湄公河次区域部分边境地区疟疾/登革热跨境联防联控试点合作项目
澜湄专项基金项目(No.2020399)
关键词
疟疾
按蚊
子孢子
巢式PCR
老挝
占巴塞省
Malaria
Anopheles
Sporozoite
Nested-PCR
Laos
Champasak Province
