摘要
本文分析了不同胎次、乳房炎及未知病牛牛乳中主要蛋白质成分,揭示了不同影响因素对牛乳中乳蛋白的影响。采用凝胶电泳(SDS-PAGE)结合液相色谱(HPLC)两种方法对不同牛乳中主要蛋白质进行分离检测,结果表明:两种方法都能够有效分离不同牛乳中的主要酪蛋白(αs-CN、β-CN和κ-CN)及乳清蛋白(α-La和β-Lg);SDS-PAGE法可得各蛋白组成及分子量;45 min内RPHPLC法可完成对牛乳中主要蛋白质定性及定量分析。
In order to reveal the influence of main milk protein on curd, the main protein components in milk from different parities, unknown disease and mastitis were analyzed. The main proteins of different milk were separated and analyzed by SDS—PAGE and HPLC. The results showed that the main casein （as-CN、β-CN and k- CN） and whey protein （a-La and β-Lg） could effectively separate from different milk by the two methods. SDS-PAGE was used to determine the protein composition and molecular weight. Qualitative and quantitative analysis of the main proteins were completed by the RP—HPLC method in 45 minutes.
作者
曹志军
赵雪平
刘敏
赵亚利
李洵洲
CAO Zhijun;ZHAO Xuepin;LIU Min;ZHAO Yali;LI Xunzhou(Inner Mongolia Agricultural University Vocational and Technical College,Baotou 014109)
出处
《中国乳品工业》
CAS
CSCD
北大核心
2018年第10期7-10,共4页
China Dairy Industry
基金
内蒙古自治区自然科学基金项目(2015MS0350)
关键词
不同胎次
乳蛋白
凝胶电泳
液相色谱
different parities
milk protein
gel electrophoresis
liquid chromatography
