摘要
目的研究分析Ax亚型伴抗A1抗体产生的血清学和分子生物学特点。方法收集1例A抗原减弱的样本,应用微柱凝胶法、试管凝集法和吸收放散实验等血清学方法鉴定该样本ABO血型表型,序列特异性引物-聚合酶链反应扩增ABO基因第6、7外显子,PCR产物经割胶纯化后直接测序。结果患者红细胞上存在A抗原,血清中有含抗A1抗体。直接测序检测到该样本646T> A; 681G> A; 771C> T; 829G> A突变。结论该样本646T> A; 681G> A;771C> T; 829G> A突变导致A抗原减弱,其基因型为Ax03。
Objective To study the serological and molecular biological characteristics of the Ax subtype antibody （A1）＇s production. Methods A sample with A phenotype（ moderately agglutinate by anti -A） was collected. The serological pheno- type of the sample was determined with a conventional method, and the ABO genotype was determined by sequence - specific primer polymerase ehain reaction（PCR -SSP）. Exons 6 and 7 of the ABO gene were amplified with PCR. Direct sequencing was then performed to the gel - purified PCR products. Results It is showed that A antigen was identified on red blood cells of the individual and there was anti - A1 antibody＇in their serum. There was 646T 〉 A, 6BIG 〉 A, 771C 〉 T, 829G 〉 A mutations by direct DNA sequencing. Conclusion The mutations on 646T 〉 A, 681G 〉 A, 771C 〉 T, 829G 〉 A may be the factors for weak A zphenotype.
作者
蔡雪娇
周湘静
谢作听
CAI Xue-jiao;ZHOU Xiang-jing;XIE Zuo-ting(Department of Blood Transfusion,the First Affiliated Hospital of Wenzhou Medical College,Wenzhou,Zhejiang 325000,China)
出处
《中国卫生检验杂志》
CAS
2018年第20期2459-2460,共2页
Chinese Journal of Health Laboratory Technology
基金
温州市科技局项目资助课题(Y20160523)
关键词
AX亚型
血型
基因型
Ax subtype
Blood type
Genotype
