摘要
快速准确鉴定血流感染病原菌对优化抗菌药物治疗和改善患者预后至关重要。传统的鉴定方法至少需要48 h。近年来，基于卷积神经网络的智能化的革兰染色阅片技术可自动判断阳性涂片中的病原菌种类，基质辅助激光解析电离飞行时间质谱可大大提高病原菌的鉴定速度。肽核酸探针荧光原位杂交技术也适用于阳性菌液中病原菌的快速鉴定。结合荧光原位杂交技术与细胞形态动力学分析技术的Accelerate Pheno系统既可直接鉴定阳性血培养中的病原菌，也可检测病原菌对抗生素的最低抑菌浓度。上述方法均可不经次代培养，直接、快速、准确地鉴定阳性血培养瓶中的病原菌。
It takes at least 48 hours for traditional identification of pathogens from blood stream infection. Therefore, a rapid identification is vital for optimizing antimicrobial therapy and improving the prognosis of patients. In recent years, the intelligentialized Gram staining based on convolutional neural network has been applied to automatically interpret the species of pathogens in positive smear and the matrix-assisted laser desorption/ ionization time of flight mass spectrometry （MALDI TOF-MS） can quickly identify pathogens. Furthermore, Peptide nucleic acid fluorescence in situ hybridization（PNA FISH）is applicable to the rapid identification of pathogens from positive bacteria liquid. Powered by morphokinetic cellular analysis （MCA） and fluorescence in situ hybridization （FISH）, the Accelerate Pheno system can directly identify pathogens from positive blood cultures and determine MIC of pathogens to antibiotics. The above-mentioned methods can provide a direct, rapid and accurate identification of pathogens from positive blood culture bottles without subculture.（Chin J Lab Med, 2018, 41： 790-793）
作者
王沛
Wang Pei(Department of Clinical Laboratory,the First People＇s Hospital of Jingmea,Jingmen 448000,China)
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2018年第10期790-793,共4页
Chinese Journal of Laboratory Medicine
关键词
血培养
微生物学技术
Blood cultures
Microbiological techniques
