摘要
植物的叶片是进行蒸腾作用、光合作用和呼吸作用的重要场所,也具有一定的观赏和营养价值。少数植物的叶片具有再生能力,可作为高效再生和遗传转化的受体。用叶片进行再生繁殖,具有取材方便、操作简洁、节约材料、对植株和环境影响少等优点,对科研和生产均具有重要的价值和意义,有待于进一步研究探讨。本研究从植物叶片再生的方式、再生形式和部位、不同因素的影响等方面介绍了植物叶片再生的研究进展。认为在细胞分裂素的作用下,从叶片切口通过愈伤组织间接再生不定芽的方式是最常见,但是效率较低,有待于改进;从叶片边缘和表面的叶脉上直接产生体胚和不定芽的方式极少,效率较高,是理想的方式和形式;最后,探讨了叶片再生的分子机制,并对挖掘植物叶片再生能力在科研和生产中的前景进行了展望。
Leaves of plants are important places for transpiration, photosynthesis and respiration, and some leaves have ornamental and nutritional values. The leaves of a few plants have the ability to regenerate and can be used as propagating material and receptors for high efficiency regeneration and genetic transformation, which have the advantages of convenient extraction, simple operation and less influence on plant and environment. Scientific research and production are of great value and significance, but there is no systematic induction and discussion. This paper introduces the methods of plant leaf regeneration, regeneration form and location, the influence of different factors, and the application of leaf regeneration in different plants. It is considered that under the action of cytokinin, the indirect regeneration of adventitious buds from the blade incision through the callus is the most common, but the efficiency is low, and it needs to be improved. The emergence of somatic embryos and buds on the veins on the edges and surfaces is very rare, novel and unique and efficient. It is an ideal way and form. Finally, the molecular mechanism of leaf regeneration is discussed, and the prospect of mining plant leaves as a molecular plant breeding and the industrialization oftransgenic plants is prospected.
作者
姜福星
黄远祥
周鹏
孙晓兰
赵婕
文好雨
陈其兵
Jiang Fuxing;Huang Yuanxiang;Zhou Peng;Su Xiaolan;Zhao Jie;Wen Haoyu;Chen Qibing(Institute of Garden Research,Sichuan Agriculture University,Chengdu,611130;Sichuan Tianyi Ecological Garden Group Incorporated Company,Chengdu,611130)
出处
《分子植物育种》
CAS
CSCD
北大核心
2018年第20期6832-6839,共8页
Molecular Plant Breeding
基金
四川省教育厅重点项目(15ZA0023)项目
立体绿化品种选育项目(640H1000)共同资助
关键词
植物叶片
再生能力
方式形式
再生机制
研究应用
Plant leaves
Regeneration ability
Mode and form
Regeneration mechanism
Research and application
