摘要
目的探讨抗骨髓炎片对家兔慢性骨髓炎的作用。方法采用右胫骨骨髓腔内注射金黄色葡萄球菌的方法建立家兔慢性骨髓炎模型,随机分为4组:抗骨髓炎片高、低剂量(480、240 mg/kg)组,庆大霉素(60 mg/kg)组,模型组(等体积0.5%CMC-Na);另取家兔向骨髓腔内注入生理盐水,作为假手术组(等体积0.5%CMC-Na),术后当天开始ig给药,每天1次,连续6周。术后第6周对右胫骨行X-线检查并做Laurence评分;术后第2、4、6周采血,血细胞分析仪进行白细胞计数,ELISA法测定血清溶菌酶、肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)含量。结果模型组家兔均有典型骨髓炎表现,抗骨髓炎片高、低剂量对骨髓炎症状有显著缓解,Laurence评分与模型组比较显著降低(P<0.01);与模型组比较,抗骨髓炎片高、低剂量组的白细胞计数、血清TNF-α和IL-6含量在术后2、4、6周均明显降低(P<0.01),高剂量组血清溶菌酶含量在术后2、4、6周均明显升高(P<0.01),低剂量组血清溶菌酶含量在第4、6周亦明显升高(P<0.05)。结论抗骨髓炎片对家兔慢性骨髓炎发挥显著改善作用,可能与抑制致病菌、提高机体免疫力、下调炎性因子等途径有关。
Objective To investigate the effects of Kanggu Suiyan Tablet（KST） against osteomyelitis in rabbits. Methods Osteomyelitis model of rabbits were prepared by intra-shinbone marrow injection of Staphylococcus aureus. Model rabbits were divided at random into four groups, i.e., model（equal volume 0.5% CMC-Na）, gentamicin（60 mg/kg）, KT high and low dose groups（480 and 240 mg/kg, respectively）. The rabbits were injected with saline into the bone marrow cavity as sham group（equal volume 0.5% CMC-Na）. Drugs were ig administrated after the operation once daily for six weeks. On the 6 th week of operation, right shinbone X-ray in rabbits were examined and laurence scores were calculated. Leucocyte counts were determined by blood cell analyzer, lysozyme, TNF-α, and IL-6 levels in sera were determined by ELISA on the 2 nd, 4 th, and 6 th weeks, respectively. Results Typical symptoms of osteomyelitis were identified in model group. Laurence scores in KT groups（480 and 240 mg/kg） were significantly reduced compared with that of model group（P〈0.01）. Comparing with model group, leucocyte count in KT groups were evidently increased on the 2 nd, 4 th, and 6 th week, respectively（P〈0.01）. Lysozyme levels were evidently increased on the 2 nd, 4 th, and 6 th week, respectively, in high dose group（P〈0.01） and 4 th and 6 th week, respectively, in low dose group（P〈0.05）. TNF-α and IL-6 levels in sera of KT groups were evidently decreased on the 2 nd, 4 th, and 6 th week, respectively（P〈0.05 or 0.01）. Conclusion The protective effect of KT on osteomyelitis seems to be associated with inhibition of pathogenic bacteria, improvement of immune function and downregulation of inflammatory cytokines, etc.
作者
张红果
吴芳
刘春梅
曹军平
ZHANG Hongguo1, WU Fang1, LIU Chunmei1, CAO Junping2(1. Center Pharmacy, General Hospital of Armed Police Forces, Beijing 100039, China 2. Department of Sterilization and Supply, General Hospital of Armed Police Forces, Beijing 100039, China)
出处
《药物评价研究》
CAS
2018年第8期1441-1445,共5页
Drug Evaluation Research
