摘要
目的探讨慢性复发型溃疡性结肠炎(UC)虚、实证患者血浆代谢组学特征。方法收集UC慢性复发型脾胃气虚证和大肠湿热证患者共31例及40例健康志愿者,观察两组患者基本情况,运用核磁共振氢谱(~1H-NMR)定量检测血浆代谢物含量,采用主成分分析法(PCA)、偏最小二乘-判别分析法(PLS-DA)及正交偏最小二乘-判别分析法(OPLS-DA)进行多元统计分析。结果筛选出潜在生物标志物10种,其中UC患者乙酰乙酸、丙酮水平高于健康人,乳酸、肌肽、丙氨酸、异亮氨酸则显著低于后者。慢性复发型UC大肠湿热证患者葡萄糖、苏氨酸、肌肽高于脾胃气虚证患者;缬氨酸、丙氨酸、甘氨酸低于脾胃气虚证患者。结论慢性复发型UC虚、实证患者存在较为明显的糖代谢、氨基酸代谢及脂代谢紊乱,UC辨证分型具有代谢物质基础。
Objective To discuss the plasma metabolomic characteristics in patients with ulcerative colitis（ UC） and deficiency or excessiveness patterns. Methods UC patients（ n = 31,UC group） with spleen-stomach qi deficiency pattern or large intestinal dampness-heat pattern and health volunteers（ n =40,control group） were chosen,and general conditions were observed in 2 groups. And UC group was divided into subgroup of large intestinal dampness-heat pattern（ subgroup A） and subgroup of spleenstomach qi deficiency pattern（ subgroup B）. The content of plasma metabolites was determined quantitatively by using H-nuclear magnetic resonance（1 H-NMR）. The multivariate statistical analysis was conducted by using principal component analysis（ PCA）,partial least squares-discriminant analysis（ PLS-DA） and orthogonal partial least squares-discriminant analysis（ OPLS-DA）. Results There were 10 potential biomarkers screened. The levels of acetoacetate and acetone were higher,and levels of lactic acid,carnosine,alanine and isoleucine were significantly lower in UC group than those in control group.The levels of glucose,threonine and carnosine were higher,and levels of valine,alanine and glycine were lower in UC patients with large intestinal dampness-heat pattern than those in UC patients with spleen-stomach qi deficiency pattern. Conclusion There are obviously disorder of glycometabolism,amino acid metabolism and lipid metabolism in UC patients with deficiency or excessiveness patterns. The pattern differentiation and classification of UC has a metabolite basis.
作者
王佳婕
曹云
李中峰
陈建新
王亮
黄佳钦
符欣
张忠绵
李志红
Wang Jiajie;Cao Yun;Li Zhongfeng;Chen Jianxin;Wang Liang;Huang Jiaqin;Fu Xin;zhangZhongmian;Li Zhihong(Yunnan Provincial Hospital of Traditional Chinese Medicine,Yunnan 650021,China;Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China;Department of Chemistry,Capital Normal University,Beijing 100048,China;Beijing University of Chinese Medicine,Beijing 100029,China;Hospital of Traditional Chinese Medicine of Beijing Huairou District,Beijing 101400,China)
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2018年第9期787-792,共6页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家重点研发计划资助项目(No.2017YFC1700106)
吴阶平医学基金会临床科研专项资助项目(No.320.6750.17233)~~
关键词
溃疡性结肠炎
代谢组学
核磁共振氢谱
大肠湿热证
脾胃气虚证
ulcerative colitis
metabolomics
H-nuclear magnetic resonance
large intestinal dampness-heat pattern
spleen-stomach qi deficiency pattern
