摘要
【目的】探究荧光假单胞菌与红绒盖牛肝菌共接种条件下NL-895杨植株氮代谢及矿质元素含量的响应,在养分代谢水平上揭示溶磷细菌与外生菌根菌互作对杨树生长的影响,为杨树复合菌剂的开发与应用提供理论依据。【方法】对2年生NL-895盆栽菌共接种荧光假单胞菌JW-JS1和红绒盖牛肝菌(Xc),测定氮代谢指标可溶性蛋白质含量、硝态氮含量和硝酸还原酶活性以及植株矿质元素含量。【结果】无论JW-JS1或Xc单接种,还是JW-JS1与Xc共接种均能显著提高NL-895杨树叶片可溶性蛋白、硝态氮含量和硝酸还原酶活性以及植株的矿质元素含量,且共接种处理优于各单接种处理。共接种30天后NL-895杨的可溶性蛋白质含量、硝态氮含量和硝酸还原酶活性分别比CK增长20.19%、79.99%和33.08%,共接种90天后增长76.76%、106.62%和34.86%,共接种150天后增长137.99%、50.70%和59.89%,植株内P、Mg、K、Ca、Mn和Zn含量分别比CK增长71.66%、23.67%、49.71%、22.90%、84.57%和151.56%。相关性分析发现,共接种30天后NL-895杨苗高和茎粗与硝态氮含量显著正相关(P<0.05);共接种90天后NL-895杨苗茎粗与可溶性蛋白含量显著正相关(P<0.05);共接种150天后NL-895杨苗高与硝酸还原酶活性、Mg含量、K含量显著正相关(P<0.05),NL-895杨茎粗与P含量显著正相关(P<0.05)、K含量极显著正相关(P<0.01)。【结论】荧光假单胞菌JW-JS1与红绒盖牛肝菌共接种显著改善NL-895杨的氮代谢功能和矿质元素的吸收从而促进NL-895杨的生长。
This paper aimed to explore the response of Populus×euramericana cv.‘Nanlin-895’ （NL-895）to nitrogen metabolism and mineral elements under co-inoculation with P. fluorescent and X. chrysenteron, to reveal the effects of interaction between phosphate-solubilizing bacteria and ectomycorrhizal fungi on poplar growth at the level of nutrient metabolism, so as to provide a theoretical basis for developing and exploiting the compound microbial fertilizer for poplar. 【Method】 A pot experiment was conducted to investigate the effects of co-inoculation with Pseudomonas fluorescent JW-JS1 and Xerocomus chrysenteron on the nitrogen metabolism （soluble protein contents, nitrate nitrogen contents and nitrate reductase activity） and mineral element contents of NL-895 poplar in the greenhouse. 【Result】 Both JW-JS1 or Xc single inoculation and JW-JS1 and Xc co-inoculation significantly increased the soluble protein contents, nitrate nitrogen contents and nitrate reductase activity of NL-895 Poplar, and improved its mineral element content, and then the treatment effect of co-inoculation was higher than single inoculations and the control. The soluble protein content, the nitrate nitrogen content and the nitrate reductase activity of NL-895 poplar increased 20.19%, 79.99%, and 33.08%, respectively after 30 days of co-inoculation, 76.76%, 106.62%, and 34.86% after 90 days of co-inoculation and 137.99%, 50.70%, and 59.89% after 150 days of co-inoculation, compared with the control. The contents of P, Mg, K, Ca, Mn and Zn of NL-895 Poplar increased 71.66%, 23.67%, 49.71%, 22.90%, 84.57% and 151.56%, respectively compared with the control after 150 days of co-inoculation. Correlation analysis showed that the height and stem diameter of NL-895 seedlings were positively correlated with nitrate nitrogen content after 30 days of co-inoculation （ P 〈0.05）, and the height of NL-895 seedlings was positively correlated with soluble protein content after 90 days of co-inoculation （ P 〈0.05）.The height of NL-89
作者
刘辉
吴小芹
任嘉红
陈丹
Liu Hui;Wu Xiaoqin;Ren Jiahong;Chen Dan(Co-Innovation Center for Sustainable Forestry in Southern China College of Forestry,Nanjing Forestry University Nanjing 210037;College of Environmental Science and Engineering,Anhui Normal University Wuhu 241003)
出处
《林业科学》
EI
CAS
CSCD
北大核心
2018年第10期56-63,共8页
Scientia Silvae Sinicae
基金
国家重点研发计划(2017YFD0600104)
国家林业公益性行业科研专项(201004061)
江苏高校优势学科建设工程资助项目(PAPD)
国家自然科学基金项目(31400589)
关键词
荧光假单胞菌
红绒盖牛肝菌
共接种
氮代谢
矿质元素
促生长
Pseudomonas fluorescent
Xerocomus chrysenteron
co-inoculation
nitrogen metabolism
mineral element
growth-promoting
