摘要
目的探讨结肠癌(CRC)组织KRAS基因突变情况并分析其与CRC病理特征、吸烟和饮酒的相关性。方法采用ARMS-PCR法检测82例CRC患者癌组织KRAS基因外显子2第12、13密码子基因突变情况,并分析其CRC病理特征、吸烟和饮酒情况与KRAS基因突变的相关性。结果 39.0%(32/82)患者KRAS基因突变,其中第12位密码子突变患者25例,占30.5%(25/82),第13位密码子突变患者7例,占8.5%(7/82); CRC组织KRAS基因突变与性别、肿瘤部位、肿瘤分期有关(P<0.05);与年龄、吸烟史、饮酒史、肿瘤分化程度、淋巴结转移于否、病理类型无关(P>0.05)。结论 CRC组织KRAS基因第12位密码子突变率高; KRAS基因突变多发生于女性患者、右半结肠、Ⅲ、Ⅳ期肿瘤患者。
Objective To investigate the correlation between KRAS gene mutations of CRC and pathological features,smoking and alcohol drinking. Methods The mutations of codon 12 and codon 13 of the KRAS gene exon 2 in cancer tissue of 82 patients with CRC were detected by ARMS-PCR method,and the correlation between CRC pathological features,smoking and alcohol drinking were analyzed. Results The KRAS gene mutations were found in 39.0%（ 32/82） of the patients.There were 25 patients with mutations in the codon 12,accounting for 30.5%（ 25/82）,and 7 patients with codon mutations in the codon13,accounting for 8.5%（ 7/82）.The KRAS gene mutations in CRC tissues were associated with gender,tumor location,and tumor stages（ P0.05） and not related to age,smoking history,alcohol drinking history,tumor differentiation,lymph node metastasis,and pathological types（ P〉0.05）.Conclusion The mutation rate of codon 12 in KRAS gene of CRC is high and KRAS gene mutations occur frequently in female patients,right colon,cancer patients with stage Ⅲ and Ⅳ.
作者
季硕超
刘淑真
郑德杰
陈悦
张盼
Ji Shuochao;Liu Shuzhen;Zheng Dejie;Chen Yue;Zhang Pan(Department of Internal Medicine,lFeifang Medical College,Weifang 261000,China;Department of Oncology,Weifang People＇s Hospital,Weifang 261000,China)
出处
《湖北民族学院学报（医学版）》
2018年第3期22-24,共3页
Journal of Hubei Minzu University(Medical Edition)
