摘要
目的了解安徽省江北地区猪隐孢子虫感染情况。方法 2014年10-12月从安徽省江北多地规模化猪场共采集500份新鲜猪粪样,采用基于隐孢子虫小亚单位核糖体核糖核酸基因(SSUrDNA)的巢式PCR方法进行检测,并对获得的阳性样本SSUrDNA基因进行序列分析以确定隐孢子虫虫种。结果安徽省江北地区猪隐孢子虫感染率为4.8%(24/500),其中阳性样本主要分布在潜山(40.0%)和滁州(6.3%),其他地区猪场未见隐孢子虫感染。SSUrDNA基因序列分析显示,所有隐孢子虫阳性样本均为种母猪隐孢子虫(Cryptosporidium scrofarum)。> 60日龄猪种母猪隐孢子虫感染率(9.1%)高于<30日龄(1.2%)和30~60日龄(1.0%)猪(P均<0.01)。结论安徽省江北地区规模化猪场存在隐孢子虫感染,且发现的种母猪隐孢子虫可能是人和其他动物隐孢子虫感染的来源,存在人兽共患潜在危害,应引起重视。
Objective To characterize the prevalence and species/genotypes of Cryptosporidium spp. in farmed pigs in the north of the Yangtze River in Anhui Province. Methods A total of 500 samples of pig feces were obtained from seven large.scale pig farms in the north of the Yangtze River in Anhui Province. PCR and sequences analysis of the small subunit rDNA gene were used to detect and identify the Cryptosporidium species/genotypes. Results The overall prevalence of Cryptosporidium was4.8%（24/500）. Additionally,Cryptosporidium prevalence was 40.0% in Qianshan and 6.3% in Chuzhou,respectively. No Cryp.tosporidium infection was found in other sampling areas. The DNA sequence analysis of the SSUrDNA gene revealed that all of the isolates represented C. scrofarum. The Cryptosporidium infection rate（9.1%）of pigs（ 〉60 days）was significantly higher than the rates of both pigs（〈 30 days）and pigs（30-60 days）（both P〈0.01）. Conclusion C. scrofarum in the farmed pigs in the north of the Yangtze River in Anhui Province may be a source of Cryptosporidium infection and pose a potential public health threat to humans and other animals,and therefore,the status should be paid more attention to.
作者
李文超
杨回回
阚珍珍
杨云龙
孙越洋
顾有方
陈会良
LI Wen-chao;YANG Hui-hui;KilN Zhen-zhen;YANG Yun-long;SUN Yue-yang;GU You-fang;CHEN Hui-liang(College of Animal Science,Anhui Science and Technology University,Fengyang 233100,China)
出处
《中国血吸虫病防治杂志》
CAS
CSCD
北大核心
2018年第4期420-423,464,共5页
Chinese Journal of Schistosomiasis Control
基金
安徽省科技重大专项(16030701066)
安徽省高校优秀青年人才重点项目(gxyqZD2016220)
安徽科技学院校级重点项目(ZRC2016478)
安徽科技学院重点学科项目(AKZDXK2015A04)
国家级大学生创新创业项目(201710879043)
