摘要
耐盐性是植物适应高渗透势盐碱环境的重要特征,植物耐盐机制受基因调控。随着土壤盐渍化的日益严重,耐盐基因的探寻对植物耐盐品种的选育以及农林业发展具有重要指导作用。本研究以胡杨F_1代90个不同基因型个体的愈伤组织为实验材料,分别采用含有0 mmol/L和80 mmol/L NaCl溶液浓度的愈伤增殖培养基进行连续培养。在培养第0天、第7天、第14天、第21天、第28天、第35天、第42天、第49天、第63天分别对各基因型个体构建愈伤生长的动态表型。联合简化基因组测序获得的8 305个SNP标记,利用功能作图方法定位耐盐基因。结果发现:(1)细胞周期蛋白基因CDC123和编码葡萄糖-6-磷酸脱氢酶基因G6PDH2在盐胁迫下激活表达,对促进愈伤组织在盐环境下的生长与适应起重要作用;(2) FD-GOGAT和ARI7是重要的生长调控基因,但其对生长的调控不受盐条件影响;(3)细胞色素CYP450家族的CYP71D11基因、几丁质酶基因Chitinase 2-like是盐敏感基因,盐环境下可使其生长缓慢。该结果可在细胞层面为耐盐机制的研究提供新的思路,并为耐盐品种的改良和选育提供理论指导。
Salt tolerance is an important feature for plants to adapt to high permeability and saline alkali environment, and the mechanism of salt tolerance of plants is regulated by genes. As soil salinization is becoming more and more serious, the exploration of salt tolerance genes has important role in the breeding of salt tolerant varieties and the development of agroforestry. In this study, callus with 90 genotypes in F1 generation of Populus euphratica was used as experimental material. The callus proliferation medium containing 0 mmol/L and 80 mmol/L NaC1 was used for continuous culture. After 0 d, 7 d, 14 d, 21 d, 28 d, 35 d, 42 d, 49 d and 63 d from the beginning of culture, phenotype of callus growth trait were investigated for each progeny. Combine with 8 305 SNP markers sequenced from RAD technology, fimctional mapping theory was employed to map salt tolerance genes. The results showed that the cell cyclin gene CDC123 and G6PDH2 encoded glucose-6-phosphate dehydrogenase were activated by salt stress, which promoted the growth and adaptation of callus under salt stress. FD-GOGA T and A RI7 were important growth regulatory genes, but their regulation on growth was not affected by salt conditions. The cytochrome CYP450 family gene CYP71Dll and Chitinase 2-1ike gene were important salt sensitive genes,which could make the calli grow slowly under salt stress. The results might lay a foundation for the elucidation of salt tolerance mechanism at cell level, and could provide theoretical guidance for improvement and markerassisted selection of salt tolerant varieties.
作者
王东洋
黄月娇
李欢
叶梅霞
邬荣领
Wang Dongyang;Huang Yuejiao;Li Huan;Ye Meixia;Wu Rongling(Center for Computational Biology,College of Biological Sciences and Technology,Beijing Forestry University,Beijing,100083)
出处
《分子植物育种》
CAS
CSCD
北大核心
2018年第19期6314-6325,共12页
Molecular Plant Breeding
基金
国家自然科学基金项目(31600536)
北京林业大学青年教师科学研究中长期项目(2015ZCQ-SW-06)
北京林业大学"国家级大学生创新创业训练计划"(201610022030)共同资助
关键词
胡杨
愈伤组织
功能定位
耐盐基因
Populus euphratica
Callus
Functional mapping
Salt tolerance gene
