摘要
目的:探讨恒古骨伤愈合剂(osteoking,OK)通过激活Wnt/β-catenin信号通路增加骨形成缓解OP的作用机制。方法:50只雌性树鼩随机分为5组,正常对照组正常喂养,OP模型组去除双侧卵巢每天进行生理盐水灌胃,OK低、中、高剂量组分别给予OK 0.5、1.5、3.0 mL/d灌胃。10周后,检测骨密度(BMD);处死树鼩,取血清和骨骼,ELISA法测定血清雌二醇(E_2)、骨碱性磷酸酶(BALP)、骨钙素(OC)、Ⅰ型原胶原N-端前肽(PINP)、抗酒石酸酸性磷酸酶5b(TRACP-5b)和Ⅰ型胶原交联C-末端肽(CTX)的含量;采用RT-q PCR和Western blot法检测骨组织Wnt1和β-catenin的表达水平。结果:与正常对照组比较,模型组树鼩血清E2含量和BMD下降明显(P<0.05),CTX、TRACP-5b、OC、PINP和BALP的含量均显著上升(P<0.05),经OK治疗后上述现象均改善明显,且具有剂量依赖性;RT-q PCR与Western Blotting结果显示Wnt1和β-catenin的表达水平经OK治疗后均明显升高。结论:OK能够通过增加E_2分泌、抑制骨吸收和促进骨形成增加骨密度缓解OP,其作用机制可能是通过激活Wnt/β-catenin信号通路实现的。
Objective： To explore the metabolism of enhancing bone formation and ameliorating OP of OK via the Wnt/β-catenin signal pathway in the castration OP tree shrew models. Methods： 50 female experimental tree shrews were randomly divided into normal control group,OP model group animals were removed bilateral ovaries and received saline by gavage daily,the tree shrews in OK-L,OK-M and OK-H groups were given OK 0. 5,1. 5 and 3. 0 mL/d by gavage for 10 weeks,determining BMD,all the tree shrews were sacrificed and serum and bone were collected,ELISA was employed to determine the concentrations of E_2,BALP,OC,PINP,TRACP-5 b and CTX; RT-q PCR and Western blotting were used to detect the expression levels of Wnt1 and β-catenin. Results： Compared with the normal control group,the serum E_2 level and BMD decreased significantly（ P〈0. 05） in the OP group,CTX,TRACP 5 b,OC,PINP and BALP levels were significantly increased（ P〈0. 05）,after intervened with OK,all the above indexes were improved obviously,and the effect took the shape of a dose-dependent manner; RT-q PCR and Western Blotting results showed that the expressions of Wnt1 and β-catenin were up-regulated after intervened with OK. Conclusion： OK can increase the BMD to alleviate OP by raising the secretion of E_2,inhibiting bone resorption and promoting bone formation, the mechanism is relate to the activation of Wnt/β-catenin signaling pathway probably.
作者
郑红
李进涛
赵宏斌
角建林
ZHENG Hong;LI Jintao;ZHAO Hongbin;JIAO Jianlin(Kunming Medical University,Kunming,Yunnan650500;First Affiliated Hospital of Kunming Medical University,Kunming,Yunnan 650032)
出处
《中国中医药科技》
CAS
2018年第5期644-648,共5页
Chinese Journal of Traditional Medical Science and Technology
基金
国家自然科学基金地区科学基金项目(81460647)
云南省科技厅-昆明医科大学应用基础研究联合专项项目(2014FB033)
