摘要
目的探讨微小RNA-502-3p(miR-502-3p)在肾癌组织中的表达及对肾癌细胞迁移、增殖的影响。方法采用荧光定量PCR检测30例肾癌及癌旁正常组织中miR-502-3p的表达,分析其与患者临床病理特征的关系。培养786-O肾癌细胞,采用miR-502-3p抑制剂(下调组)、功能类似物(过表达组)或乱序(对照组)转染细胞,Transwell实验以及划痕实验检测肾癌细胞迁移能力,CCK-8实验检测肾癌细胞增殖能力。结果肾癌组织miR-502-3p表达低于癌旁组织(P<0.01)。miR-502-3p表达水平与患者年龄、性别、病理类型和临床分期无关(P>0.05)。与对照组相比,下调组细胞迁移细胞数增加,过表达组迁移细胞数量减少(P<0.01或P<0.05)。与对照组相比,下调组肾癌细胞增殖能力增强,过表达组增殖能力降低(P<0.01)。结论 miR-502-3p在肾癌组织中低表达。miR-502-3p能抑制肾癌细胞的迁移和增殖,可能与肾癌的发生和发展有关。
Objective To investigate the expression of microRNA-502-3 p（miR-502-3p）in renal carcinoma tissues and its effect on the migration and proliferation of renal carcinoma cells.Methods The expression of miR-502-3p in 30 cases of renal carcinoma and tumor-adjacent normal tissues were detected by fluorescence quantitative PCR.The relationship of miR-502-3p expression and clinicopathological characteristics was analyzed.The renal carcinoma cell 786-O was cultured and transfected by miR-502-3p inhibitor（group A）,functional analogue（group B）and disorder（group C）.The cell migration and proliferation were detected.Results The expression of miR-502-3p in renal carcinoma tissues was lower than that in tumor-adjacent normal tissues（P〈0.01）.The expression of miR-502-3p was not related to the age,gender,pathological type and clinical stages（P〉0.05）.Compared to group C,the number of migration cell and proliferation ability were higher in group A and lower in group B（P〈0.01 or P〈0.05）.Conclusion The expression of miR-502-3p is downregulated in renal carcinoma tissues.miR-502-3p can inhibit the migration and proliferation of renal carcinoma cells,indicating that it may be involved in the occurrence and development of renal carcinoma.
作者
陈俊泳
韩耕宇
徐雅
陈星星
聂奇伟
张征荣
邓健
谢群
CHEN Junyong;HAN Gengyu;XU Ya(Department of Urology,Zhuhai People＇s Hospital,Zhuhai 519000,CHINA)
出处
《江苏医药》
CAS
2018年第9期999-1003,共5页
Jiangsu Medical Journal
基金
珠海市医学科研基金(2016J030)
