摘要
谷子作为我国的传统优势作物,具有抗旱、耐瘠薄、基因组小等突出优势,已逐渐发展为研究禾本科作物新的模式作物。利用高通量RNA-seq技术,我们对干旱胁迫6 h和6 d的谷子进行转录组测定,筛选表达差异基因。结果显示,干旱胁迫后的差异基因主要富集在生物学通路及代谢通路中,其中编码蛋白激酶、磷酸酶、信号转导组分、转录因子、功能蛋白等基因的表达变化较明显。尤其在干旱胁迫6 d后,谷子中E3泛素连接酶介导的泛素蛋白酶体途径被明显激活。该研究对于筛选谷子抗旱基因进而揭示谷子的抗旱机制提供了重要依据。
Foxtail millet [ Setaria italica （ L. ） Beauv. ] is an ancient major food and fodder cereal crops in China. It has been regarded as an ideal model crop for studying Poaceae plants with its significant characters of drought and balTen - tolerant and small - genome size. Using high throughput of RNA - Seq technology, a set of drought regulated genes were detemreined after 6 - h and 6 - day reatments and the differentially expressed genes were screened out. The results showed that the drought - responsive genes were mainly enriched in biological and metabolic processes, and the expression of genes coding protein kinase and phosphatase, component of signaling transduction, transcription factors, and functional proteins changed significantly. E3 ubiquitinprotein ligase participated ubiquitin proteasome system （UPS） was dramatically activated after drought treated for 6 days. This research would be in aid of screening candidate genes of drought resistance, and elucidate the drought tolerance mechanisms of foxtail millet.
作者
王庆国
李臻
管延安
刘炜
潘教文
Wang Qingguo;Li Zhen;Guan Yan＇an;Liu Wei;Pan Jiaowen(Biotechnology Research Center,Shandong Acadamy of Agricuhural Sciences/Shandong Provincial Key LaboratoU of Crop Genetic Improvement,Ecology and Ph,ysiology,Jinan 250100,China;Crop Research Institute,Shandong Acadamy of Agricuhural Seiences,Jinan 250100,China)
出处
《山东农业科学》
2018年第10期6-12,共7页
Shandong Agricultural Sciences
基金
山东省重点研发计划项目"耐盐碱特色经济作物分子机理研究"(2016ZDJS10A03-05)
山东省重大科技专项"耐盐碱作物新品种培育改良关键技术研究"项目(2015ZDGS03001-2)
山东省自然科学基金面上项目(ZR2016CM23)
山东省农业科学院青年英才计划重点扶持类项目(2016-2018)
关键词
谷子
干旱胁迫
转录组
基因表达
Foxtail millet
Drought stress
Transcriptome
Gene expression
