摘要
本文建立了环介导等温扩增技术(Loop-mediated isothermal amplification,LAMP)快速检测化妆品中铜绿假单胞菌的方法。采用铜绿假单胞菌外膜蛋白oprI基因保守序列引物,评价检测铜绿假单胞菌灵敏度和特异性,并与普通PCR相比,检测人工污染样品中的铜绿假单胞菌。结果显示,LAMP检测铜绿假单胞菌的灵敏度为62. 5 pg/μL,而且特异性高,人工污染样品中的检出限为102cfu/m L,比PCR检测灵敏度高10倍。该方法具有灵敏度高、特异性好、操作简便、耗时短,可用于化妆品中铜绿假单胞菌的快速检测。
A rapid method for detecting Pseudomonas aeruginosa in cosmetics by loop-mediated isothermal amplification （LAMP） was established. The sensitivity and specificity of detecting Pseudornonas aeruginosa were evaluated by using conserved primers of oprI gene of Pseudomonas aeruginosa outer membrane protein gene. Compared with the common PCR, Pseudornonas aeruginosa was detected in artificially contaminated samples. The results showed that the sensitivity of LAMP for detecting Pseudomonas aeruginosa was 62.5 pg/μL, and the specificity was high. The detection limit in artificially contaminated samples was 10^2 CFU/mL, which was 10 times higher than that of PCR. This method had the advantages of high sensitivity, good specificity, simple operation and short time consuming. It could be used for the rapid detection of Pseudomonas aeruginosa in cosmetics.
作者
文霞
刘静霞
张淑瑶
杨秀茳
谢小保
WEN Xia;LIU Jingxia;ZHANG Shuyao;YANG Xiujiang;XIE Xiaobao(Guangdong Institute of Microbiology,Guangdong Detection Center of Microbiology,State Key Laboratory of Applied Microbiology Southern China,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Open Laboratory of Applied Microbiology,Guangdong,Guangzhou 510070)
出处
《工业微生物》
CAS
2018年第5期37-41,共5页
Industrial Microbiology
基金
广东省科技计划项目(2014A040401054)
关键词
化妆品
环介导等温扩增技术
铜绿假单胞菌
cosmetics
loop-mediated isothermal amplification
Pseudomonas aeruginosa
