摘要
目的:探讨miR-101对口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)细胞SPOP基因的靶向关系及其对细胞迁移的影响。方法:使用Real time-PCR技术检测miR-101在OSCC细胞中的表达;利用生物信息分析预测SPOP可能是miR-101靶基因,将miR-101转染至Tca-8113细胞株,通过增强或者抑制miR-101的表达,利用蛋白质印迹实验检测SPOP表达,从而判断两者靶向关系;利用细胞迁移试验,通过miR-101表达强弱改变,判断Tca-8113细胞株迁移能力的变化情况。结果:miR-101在三个OSCC细胞株中表达均降低;在Tca-8113细胞株中增强miR-101表达,则SPOP表达下降,细胞迁移能力减弱;抑制miR-101表达,则SPOP表达升高,细胞迁移能力增强。结论:miR-101对OSCC细胞SPOP有靶向抑制作用,并且可以抑制OSCC细胞转移。
Objective To investigate the functional roles of miR-101 on SPOP gene expression and cell migration in oral squamous cell carcinoma（OSCC）. Methods： The expression levels of miR-101 in human OSCC cell lines（Cal-27,SCC-15 and Tca-8113）were detected by Real time-PCR.Bioinformatics analysis revealed SPOP as a candidate target genes of miR-101.After transfecting miR-101 of mimics or inhibitors in Tca-8113 cells,expression of SPOP was examined with Western blot,and cell migration was evaluated with wound assay. Results： The miR-101 expression was significantly lower in OSCC cell lines than that in normal oral mucosal cell.Western blot assay showed SPOP expression was decreased when miR-101 was transfected,while SPOP expression was increased when miR-101 expression was inhibited in Tca-8113 cells.Wound assay demonstrated that migration abilities of Tca-8113 cell was significantly suppressed by up-regulating miR-101 expression. Conclusion： In OSCC,miR-101 can suppress SPOP expression and tumor migration by targeting SPOP.
作者
许志鹏
陈琳
李建虎
高岭
郅克谦
Xu Zhipeng;Chen Lin;Li Jianhu;Gao Ling;Zhi Keqian(Stomatology Department;Pathology Department,Shaanxi Provincial People＇s Hospital,Shaanxi Xi＇an 710068,China;StomatologyDepartment,the Affiliated Hospital of Qingdao University,Shandong Qingdao 266500,China.)
出处
《现代肿瘤医学》
CAS
2018年第21期3358-3363,共6页
Journal of Modern Oncology
基金
陕西省社会发展科技攻关项目(编号:2015SF159)
