摘要
通过电化学还原法制备纳米Cu_2O/还原石墨烯复合修饰电极(Cu_2O-reducedgrapheneoxidenanocomposite modified glass carbon electrode,Cu_2O-RGO/GCE),用于多巴胺(dopamine,DA)的检测。采用扫描电镜和X-射线粉末衍射仪对不同修饰电极进行微观形貌表征,进一步优化电化学还原条件和测定DA实验条件。此外,通过循环伏安法考察DA在裸电极及RGO或Cu_2O-RGO上的电化学响应。Cu_2O-RGO/GCE实现抗坏血酸(ascorbic acid,AA)、DA和尿酸(uric acid,UA)氧化峰的有效分离,AA-DA和DA-UA的氧化峰电位差分别为204 mV和144 mV。该修饰电极检测的线性范围为1×10^(-8)～1×10^(-6) mol/L和1×10^(-6)～8×10^(-5) mol/L,检出限为6.0×10^(-9) mol/L。该修饰电极用于盐酸多巴胺注射液和血清中DA的含量测定,获得结果较好。
Cu2O-reduced graphene oxide nanocomposite （Cu2O-RGO） was applied to modify glassy carbon electrodes （GCE） in order to determine dopamine （DA）. The microstructure of the modified electrodes was characterized by scanning electron microscope （SEM） and X-ray differentiation （XRD）. Then the electrochemical reduction conditions for preparing Cu2O-RGO/GCE and the experimental conditions for detecting DA were optimized. The electrochemical behavior of DA on the bare electrode and RGO or Cu2O-RGO modified electrodes was also investigated in pH 3.5 phosphate buffer solution （PBS） by cyclic voltammetry. The results showed that the oxidation peaks of ascorbic acid （AA）, DA and uric acid （UA） could be well separated and the peak-to-peak potential separations between DA and AA and UA were 204 and 144 mV, respectively. Moreover, the linear response ranges for the determination of DA were 1 × 10^（-8）–1 × 10^（-6） mol/L and 1 × 10^（-6） –8 × 10^（-5） mol/L and the limit of detection （LOD） was 6.0 × 10^（-9） mol/L （RSN = 3）. The proposed method was further applied to determine DA in dopamine injections and serum with satisfactory results.
作者
贺全国
李广利
刘军
刘晓鹏
梁静
邓培红
HE Quanguo;LI Guangli;LIU Jun;LIU Xiaopeng;LIANG Jing;DENG Peihong(College of Life Sciences and Chemistry,Hunan University of Technology,Zhuzhou 412007,China;Department of Chemistry and Material Science,Hengyang Normal University,Hengyang 421008,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2018年第20期308-314,共7页
Food Science
基金
国家自然科学基金青年科学基金项目(61703152)
湖南省自然科学基金青年基金项目(2016JJ4010
2018JJ3134)
株洲市科技计划项目(201707-201806)
关键词
纳米Cu2O
还原石墨烯
修饰电极
多巴胺
电催化氧化
nano-cuprous oxide
reduced graphene oxide
modified electrode
dopamine
electrocatalytic oxidation
