摘要
采用酸碱提取法提取总碱,酸性染料比色法测定蒙古扁桃药材总生物碱的含量。经石油醚脱脂后,用乙醇提取得总生物碱,以硫酸阿托品为对照品,通过酸性染料比色法测定其总生物碱的含量。结果表明,硫酸阿托品在0～12μg/m L的浓度范围内含量与吸光度呈良好的线性关系。结果测得总生物碱含量为0. 1820 mg/g,加样回收率为98. 36%,RSD=0. 85%,说明此方法方法操作简便、稳定且重复性好,可作为蒙古扁桃药材生物碱的含量测定方法也为其资源开发利用提供科学依据。
Total alkali was extracted by acid-base extraction method, and total alkaloids of Amygdalus mongolica were determined by acid dye colorimetry. After degreasing with petroleum ether, total alkaloids were extracted with ethanol, and atropine sulfate was used as a reference substance, and the total alkaloid content was determined by acid dye colorimetry. The results showed that the content of atropine sulfate in the concentration range of 0~12 μg/mL showed a good linear relationship with the absorbance. The results showed that the total alkaloid content was 0.1820 mg/g, the recovery rate was 98.36%, and the RSD was 0.85%, indicating that the method was simple, stable and reproducible. It can be used as a method for determination of alkaloids in Amygdalus mongolica . It also provided a scientific basis for the development and utilization of its resources.
作者
贾小叶
高晨
刘庆
白迎春
石松利
JIA Xiao-ye;GAO Chen;LIU Qing;BAI Ying-chun;SHI Song-li(Baotou Medical College,Inner Mongolia University of Science and Technology,Inner Mongolia Baotou 014040,China)
出处
《广州化工》
CAS
2018年第19期100-102,共3页
GuangZhou Chemical Industry
基金
国家自然科学基金(81760782
81641137)
内蒙古自治区高等学校青年科技英才支持计划(NJYT-15-B01)
内蒙古自然科学基金项目(2015MS0808)
关键词
酸碱提取法
酸性染料比色法
蒙古扁桃
总生物碱
acid-base extraction
acid dye colorimetcy
Amygdalus mongolica
total alkaloid
