摘要
目的探讨生物素化微细纤维肽修饰胰岛素样生长因子-1(IGF-1)对急性心肌梗死(AMI)兔血清炎性因子的影响。方法将28只新西兰白兔随机分为对照组和实验1组、实验2组、实验3组,每组7只。对照组行假手术,实验1、2、3组建立AMI模型。建模成功后,实验1组注射生物素化微细纤维肽修饰的IGF-1,实验2组注射未修饰的IGF-1,对照组和实验3组注射等剂量的生理盐水。干预1周后,采用酶联免疫吸附试验(ELISA)法检测血清白细胞介素-6(IL-6)、超敏C-反应蛋白(hs-CRP)、肿瘤坏死因子-α(TNF-α)的水平。结果干预1周后,与对照组比较,实验1、2、3组血清IL-6、hs-CRP、TNF-α水平均显著升高(均P<0.05);与实验3组比较,实验1、2组血清IL-6、hs-CRP、TNF-α水平均显著降低(均P<0.05);与实验2组比较,实验1组血清IL-6、hs-CRP、TNF-α水平均显著降低(均P<0.05)。结论 IGF-1和生物素化微细纤维肽修饰的IGF-1均能够显著降低AMI兔炎性因子的水平,但生物素化微细纤维肽修饰的IGF-1效果更优。
Objective To explore the influence of biological microfiber peptides modified insu lin like growth factor I（IGF-1） on serum inflammatory factors in rabbits with acute myocardial infarction（AMI）.Methods Twenty eight New Zealand white rabbits were randomly divided into control group, experimental group 1, experimental group 2 and experimental group 3 ,with 7 tab bits in each group.The sham operation was performed in control group. The AMI was induced in experimental groups.After model establishment,the experimental group 1 was injected with bio logical microfiber peptides modified IGF-1,and the experimental group 2 with unmodified IGF-1. Both control group and experimental group 3 were given the same volume of normal saline.Serum levels of interleukin 6（IL-6）,high sensitivity C reactive protein（hs CRP） and tumor necrosis fac tot a（TNF-a） were detected by ELISA after 1 week of intervention.Results Compared with con trol group,serum IL-6, hs CRP and TNF-a levels increased in experimental groups（P〈0.05）. Compared with experimental group 2,the levels of IL-6, hs CRP and TNF-a increased in experi mental group 3,but decreased in experimental group l（P〈0.05）.Conclusion Both IGF-1 and biological microfiber peptides modified IGF-1 can significantly reduce the levels tory factors in AMI rabbits.However, biological microfiber peptides modified unmodified IGF-1 for treating AMI. IGF-1 is superior to
作者
胡云华
肖祖克
HU Yun-hua;XIAO Zu-ke(Cadre Department of Cardiology,Jiang~i Provincial People ~ s Hospital,Nanchang 330006,China)
出处
《南昌大学学报（医学版）》
CAS
2018年第4期19-21,25,共4页
Journal of Nanchang University：Medical Sciences
关键词
生物素化微细纤维肽修饰IGF-1
心肌梗死
急性
炎性因子
血清
动物
实验
白兔
biological microfiber peptides modified IGF-1
myocardial infarction,acute
inflammatory factors
serum
animals
laboratory
white rabbits
