摘要
目的:研究尾静脉注射骨形成蛋白7(BMP-7)过表达质粒在小鼠心、肝、肺和肾脏组织中的表达及其安全性。方法:将30只小鼠随机分为正常组(NC组)、空载质粒组(NC+Vector组)以及BMP-7过表达质粒组(NC+BMP-7组),NC+BMP-7组和NC+Vector组分别通过尾静脉注射BMP-7过表达质粒或空载质粒,每周1次共注射6周,6周后处死小鼠取动脉血,检测血清中小鼠胆固醇、甘油三酯和血糖含量,收集小鼠心、肝、肺和肾脏组织,采用HE染色观察小鼠肝脏和肾脏组织形态变化,试剂盒比色法检测肝组织中天门冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)活力,Western Blot检测各组织中BMP-7表达。结果:与NC组比较,NC+Vector组和NC+BMP-7组小鼠胆固醇、甘油三酯和血糖无明显变化,肝、肾组织显微镜观察无明显形态学改变,肝组织中AST和ALT的活力无明显增高,但小鼠心、肝、肺和肾脏组织中BMP-7蛋白明显增加,差异有统计学意义(P <0. 05)。结论:通过尾静脉注射BMP-7过表达质粒可以在小鼠心、肝、肺和肾脏中高表达,且不具有明显的肝、肾毒性。
Objective： To study the expression and safety of bone morphogenetic protein 7 （BMP-7） in rat hearts, livers, lungs and kidney tissues. Methods： 30 mice were randomly divided into normal group （NC group）, no-load plasmid group （NC ＋ Vector group） and BMP-7 expression plasmid group （NC ＋ BMP-7 group）. By injecting the rat tail vein with BMP-7 expression plasmid and empty plasmid once a week for 6 weeks in NC ＋ BMP-7 group and NC ＋ Vector group respeetively, the mice were executed six weeks later to take blood and take hearts, livers, lungs and kidney tissues. The serum was used to detect cholesterol, triglyeerides, and blood sugar in mice. HE staining was used to observe the changes in livers and kidney tissues in mice. The reagent box colorimetry was used to detect the activity of alanine aminotransferase（ALT） and glutamate aminotransferase （AST） in liver tissues. The expression of BMP-7 in various organizations was detected by Western Blot. Results： There were no significant changes in cholesterol, triglycerides and blood sugar in NC ＋ Vector group and NC ＋ BMP-7 group after plasmid injection, and there was no obvious change in morphology, and no increase in aminotransferase and aminotransferase. BMP-7 protein was significantly increased in the hearts, livers, lungs and kidney tissues of mice. Conclusion ： BMP-7 expression plasmid can be expressed high in the hearts, livers, lungs and kidneys of mice and There is no obvious toxicity of livers or kidneys.
作者
彭伟
刘慧铭
梁露群
伍聪聪
徐卫卫
周星丞
王圆圆
刘丽荣
桂华珍
郭兵
PENG Wei;LIU Huiming;LIANG Luqun;WU Congcong;XU Weiwei;ZHOU Xlngcheng;WANG Yuanyuan;LIU Lirong;GUI Huazhen;GUO Bing(Department of Pathophysiology,School of Basic Medicine,Guizhou Medical University,Guiyang 550025,Guizhou,China;Guizhou Prosinical Key Laboratory of Pathogensis ＆ Grug Researeh on Common Chronic Diseases,Guizhou Medical University,Guiyang 550025,Guizhou,China;Department of Clinical Hematology,School of Medical Diagnositics,Guizhou Medical University,Guiyang 550025,Guizhou,China;Department of Muhimedia Teaching and Researeh,School of Basic Medicine,Guizhou Medical University,Guiyang 550025,Guizhou,China)
出处
《贵州医科大学学报》
CAS
2018年第10期1192-1197,共6页
Journal of Guizhou Medical University
基金
国家自然科学基金资助项目(81760131)
贵州省科学技术基金[黔科合基础(2016)1117]
贵州省教育厅青年科技人才成长项目[黔教合KY字(2016)150]
