摘要
目的探讨Fra-2(Fos-related antigen-2)基因在喉鳞状细胞癌(LSCC)中表达情况。方法采用免疫组化SP法及逆转录-多聚酶链反应(RT-PCR)检测47例LSCC组织和21例癌旁正常组织中Fra-2基因蛋白及mRNA水平的表达情况,在癌组织当中针对这些基因表达和相关临床病理参数之间存在的关系进行分析。结果 Fra-2基因蛋白水平在LSCC阳性表达率高于癌旁正常组织(P<0.01),其表达与临床分期、淋巴结转移、吸烟均相关,与病理分级、年龄、解剖分区均无关;mRNA水平表达量高于癌旁正常组织(P<0.01),此水平表达仅与病理分级相关,与临床分期、淋巴结转移、吸烟、年龄、解剖分区无关。结论喉鳞状细胞癌中Fra-2基因在蛋白及mRNA水平均表达增高,此基因活化表达增加可能促进喉癌的发生与发展。
Objective To investigate expressions of Fra-2 in laryngeal squamous cell carcinoma.Methods Using immunohistochemistry and reverse transcription-polymer chain reaction（RT-PCR）detecting 47 cases of laryngeal squamous carcinoma tissue and 21 cases of para-carcinoma tissues,in the level of protein and mRNA.We analyzed the relationship of the genes expression and clinopathological parameters.Results The level of protein positive expression of Fra-2 in laryngeal squamous cancer tissue are higher than adjacent tissues（P〈0.01）.Protein levels of Fra-2 in laryngeal squamous cancer tissue are significant correlation with the clinical stages,lymph metastasis,smoking,but not with the pathological grade,age,anatomic site.The mRNA level expression of this genes in laryngeal squamous cancer are higher than adjacent tissues（P〈0.01）.The expressing of Fra-2 gene in mRNA level was relevent to the pathologic stage.wasn＇t relevent to clinical stages,lymph metastasis,smoking,age,anatomic site.Conclusion In laryngeal squamous cancer,The expression of Fra-2 gene both in protein and mRNA level were increasd.The activation and expression of Fra-2 may play a role in laryngeal cancer.
作者
张云龙
宋晓飞
段云静
赵辉明
赵瑞力
ZHANG Yun-long;SONG Xiao-fei;DUAN Yun-jing(The Third department of Intensive Care Unit,the First Hospital of Shijiazhuang,Shijiazhuang 050011,China)
出处
《中国实验诊断学》
2018年第9期1493-1496,共4页
Chinese Journal of Laboratory Diagnosis
