摘要
目的比较陆眠宁Ⅱ、乌拉坦全身麻醉对家兔酸碱平衡重要指标的影响。方法成年健康新西兰大白兔40只,雌雄各半,随机分为2组,分别为陆眠宁Ⅱ组和乌拉坦组。陆眠宁Ⅱ组采用低剂量多频次肌注给药:总剂量0.3~0.4 mL·kg^(-1)。乌拉坦组采用质量分数25%乌拉坦按5 mL·kg^(-1)耳缘静脉给药,麻醉前耳缘动脉采血测定血气指标,麻醉后观察显效时间、维持时间及动物死亡情况,股动脉采血测定血气指标。比较两种麻醉药对酸碱平衡重要指标pH、碱剩余(BE)、二氧化碳分压(PaCO_2)及HCO_3^-的影响。结果陆眠宁Ⅱ有较好的镇静麻醉效果,呼吸抑制稍明显,但不易造成动物死亡。陆眠宁Ⅱ麻醉后家兔pH(7.35±0.04),BE(6.8±3.49)mmol·L^(-1),PaCO_2(58.32±8.45)mm Hg,HCO_3^-(32±3.02)mmol·L^(-1);乌拉坦麻醉后家兔pH(7.36±0.04),BE(-5.8±3.12)mmol·L^(-1),PaCO_2(33.44±7.05)mm Hg,HCO_3^-(16.23±3.15)mmol·L^(-1),陆眠宁Ⅱ组与正常对照相比,pH无明显差异(P>0.05),BE、PaCO_2及HCO_3^-显著高于正常组(P<0.01);乌拉坦组与正常对照相比,各项指标均无显著性差异(P>0.05)。结论本研究获得了陆眠宁Ⅱ麻醉对家兔酸碱平衡重要指标影响的实验资料,为实验教学、科研及兽医临床提供参考。
Objective To compare the effects on major indexes of acid-base balance between Lu Mianning 11 and 25 % urethane. Methods A total of 40 adult healthy New Zealand white rabbits with half male and half female were randomly divided into two groups, Lu Mianning 11 and urethane. As control, before anesthesia, blood samples were collected from auricular arteria for blood gas analysis. Twenty of them were intravenously injected with 5 mL. kg-t of 25% urethane, and twenty rabbits were given intramuscular injection of Lu Mianning I1 by multiple low-dosing： the total dose was 0. 3 - 0. 4 mL. kg - 1. After the general anesthesia, the acid-base balance disorder experiment was carried out. The onset time, duration time and the death of animals were observed. The differences of pH, BE, HCO3- and PaCO2 in the acid-base balance test were compared. Results Lu Mianning 11 had a good effect of sedation anesthesia, and the respiratory inhibition was obvious, while it was not easy to cause the death of the animals. After Lu Mianning il anesthesia,the pH,BE, HCO3- and PaCO2 were （ 7.35 ± 0. 04 ）, （ 6. 8 ± 3.49 ） mmol. L-1 , （ 32 ± 3.02 ） mmol. L-1, and （ 58.32 ± 8.45 ） mmHg; After urethane anesthesia, the pH, BE, HCO3- and PaCO2 were （7.36±0.04） ,（ -5. 8±3.12） mmol.L-1, （16.23±3.15） mmol. L-1,and （33.44±7.05） mmHg. Compared with control, there was no significant difference in pH （ P 〉 0. 05 ）, while significant increases were observed in PaCO2 ,BE and HCO3- （P 〈 0. 01 ） of Lu Mianning 1I group. All indexes of urethane have no difference compared to control （P 〉 0.05 ）. Conclusion This study obtained experimental data of major indexes on acid base balance experiment in rabbits, which provide reference for experimental teaching, scientific research and veterinary clinic.
作者
冯雪梅
倪雯雯
崔永耀
胡优敏
FENG Xuemei;NI Wenwen;CUI Yongyao;HU Youmin(Basic Medical Experimental Teaching Center,Shanghai Jiaotong University School of Medicine,Shanghai 200025,China)
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2018年第9期791-794,共4页
Journal of Shenyang Pharmaceutical University
基金
国家自然科学基金资助项目(81370144)
关键词
陆眠宁Ⅱ
乌拉坦
麻醉
酸碱平衡
Lu Mianning 1I
urethane
anesthesia
acid-base balance
