摘要
目的完成鼠疫耶尔森氏菌重要功能蛋白caf 1的精细线性抗原表位鉴定。方法利用DNA重组技术获得重组蛋白rcaf 1,免疫新西兰兔制备抗rcaf 1多克隆抗体;使用DNA Star-protean软件及生物网站http://www.cbs.dtu.dk/services/Signal P/预测rcaf 1的抗原表位,并通过基因工程技术获得连接KLH载体蛋白的多肽;使用ELISA方法鉴定预测的精细抗原表位。结果对鼠疫耶尔森氏菌重要功能蛋白caf 1预测的精细线性抗原表位,经ELISA法检测,其中多肽片段P1为弱阳性、P2为阳性,具有较好的免疫原性。结论针对鼠疫耶尔森菌重要功能蛋白caf 1抗原表位的预测是准确的,为鼠疫潜在诊断靶点及新型疫苗的选择提供了可靠依据。
Objective To finish the identification of fine linear antigen epitope of important function protein caf 1 of Yersinia pestis. Methods Recombinant protein rcaf 1 was obtained by DNA recombination technology, and anti-rcaf 1 polyclonal antibody was prepared by immunizing New Zealand rabbits. The antigen epitope of caf 1 was predicted with the DNA Star-protean software and at the biological website http：//www.cbs.dtu.dk/services/Signal P/. The polypeptide connected to KLH carrier protein was obtained by genetic engineering. Identification of the predicted caf 1 fine epitopes was conducted with the method of enzyme-linked immunosorbent double antibody sandwich assay. Results Two fine antigen epitopes of the predicted were tested by ELISA experiments. P1 was weak positive and P2 was positive with good immunogenicity.Conclusions The prediction of the antigen epitopes of the important function protein caf 1 of Yersinia pestis is accurate and provides a reliable basis for the selection of potential diagnosis targets and new vaccines of plague.
作者
郭荣
阿布力米提.莫明
刘希佳
孙素荣
GUO Rong;Abulimiti MOMING;LIU Xi-jia;SUN Su-rong(The Center for Disease Control and Prevention of Xinjiang Uygur Autonomous Region Urumqi,Xinjiang 830002,China)
出处
《疾病预防控制通报》
2018年第4期8-10,33,共4页
Bulletin of Disease Control & Prevention(China)
基金
国家自然科学基金(81760365)
科技基础性工作专项重点项目计划(2013FY113500)
