摘要
目的通过观察小鼠体内镍离子含量、皮肤、细胞因子及肠道菌群的变化,评价镍对小鼠免疫功能和肠道菌群的影响。方法雌性ICR小鼠30只,随机分为高剂量组、低剂量组和对照组。采用原子吸收分光光度法测定肾脏镍含量、耳朵厚度评价镍攻击的反应、ELISA测细胞因子、16SrDNA测序法评价肠道菌群。结果与对照组相比,高剂量组肾脏中镍含量明显升高,分别为(0.33±0.11)和(2.61±1.29),差异有统计学意义(P=0.016)。趋化因子CXCL1与耳朵厚度在各组之间差异无统计学意义(P=0.286、0.117)。和对照组相比,部分肠道菌群Lachnospiraceae_NK4A136_group毛螺菌科(P=0.002)、Lachnospiraceae_UCG-001毛螺菌科(P=0.042)、Bacteroides拟杆菌科(P=0.016),以及Intestinimonas属(P=0.018)在高剂量组增加,差异有统计学意义。结论经饮水镍暴露小鼠,会导致镍在体内沉积。镍暴露可以导致肠道菌群发生改变。实验结果未显示镍暴露可以引发先天性免疫相关细胞因子变化以及镍特异性细胞免疫应答。
Objective To observe the effects of nickel exposure on murine immune response and intestinal bacteria flora. Methods 30 female ICR mice were randomly divided into three groups and were provided with drinking water containing purified nickel at 0（controls）,9μmol/L（low dose）and 380μmol/L（high dose）concentration.Atomic absorption spectrophotometry was used to determine nickel content in kidneys.The nickel induced allergic response was evaluated by measuring murine ear thickness.Cytokine levels were measured by ELISA.Intestinal microflora was defined by 16 SrDNA sequencing. Results The level of nickel in murine kidneys was significantly higher among mice in high dose group compared to mice in control group（2.61±1.29 vs 0.33±0.11,P=0.016）.There were no significant differences in chemokine CXCL1 level（P=0.286）and ear thickness（P=0.117）between groups.Compared to mice in control group,the relative abundances of certain intestinal bacteria including LachnospiraceaeNK4 A136group（P=0.002）,LachnospiraceaeUCG-001（P=0.042）,Bacteroides（P=0.016）and Intestinimonas（P=0.018）increased significantly in mice exposed to high nickel concentration. Conclusions Nickel exposure through drinking water can lead to nickel deposition in vivo.Nickel exposure can change intestinal microbioda.However,exposure to nickel in drinking water fails to influence murine innate immune response as well as specific cellular immune response.
作者
陈清玉
吴天遂
李知同
李军
CHEN Qing yu;WU Tian-sui;LI Zhi-tong;LI Jun(The High School Affiliated to Renmin University of China,Beijing 100080,China)
出处
《中国预防医学杂志》
CAS
CSCD
2018年第8期573-578,共6页
Chinese Preventive Medicine
基金
国家科技部重大仪器专项项目(2011yq030114)
