摘要
采用高效液相色谱柱前衍生法测定酵母抽提物中谷氨酸的含量,以苯异硫氰酸作为柱前衍生试剂,在C18柱上,弱碱性条件以保留时间定性、外标法定量。结果表明,在0.2~1.0mg/mL线性范围内,线性相关系数为0.999816,相对标准偏差小于5%,回收率在95%~110%,样品检测结果良好。该方法还可以用于其他氨基酸的检测。
The content of glutamic acid in yeast extract was determined by high pefformance liquid chromatography column derivatization. Benzo thiocyanic acid was used as a pre column derivatization reagent. On the C 18 column, the retention time was qualitative and the external standard was quantified under the condition of weak alkali. The resuits show- that in the linear range of 0.2 - 1.0mg/mL, the linear correlation coefficient is 0.999816, the relative standard deviation is less than 5%, the recovery rate is between 95% - 110%, and the sample test results ale good. This method can also be used for the detection of other amino acids.
作者
李素媛
喻玺
刘恒
余佳
余术
黄龙
赵吉媛
Li Suyuan(Three Gorges Product Quality Inspection and Testing Center,Yichang 443000,China)
出处
《安徽农学通报》
2018年第18期18-19,共2页
Anhui Agricultural Science Bulletin
关键词
高效液相色谱法
谷氨酸
柱前衍生
High perfomlance liquid chromatography （HPLC）
Clutamic acid
Pre column derivatization
