摘要
目的探讨血清γ-干扰素诱导蛋白10(IP-10)在活动性肺结核患者中的诊断价值。方法选择新疆医科大学第一附属医院2017年1-12月收治的活动性肺结核病初诊患者72例(活动性肺结核组);另将本院呼吸科同期住院的肺炎、肺癌和肺气肿等其他肺部疾病患者列为非结核呼吸病组;对照组30例,来源于同期来本院的健康体检者。采用酶联免疫吸附(ELISA)法检测3组血清IP-10,实际操作按说明书要求进行。血清T-SPOT的检测按T-SPOT.TB试剂盒操作规范进行。结果 3组IP-10和IFN-γ水平之间差异有统计学意义(P<0.05)。活动性肺结核组IP-10以及IFN-γ经检验均明显高于非结核呼吸病组和对照组(P<0.05),但健康对照组和非结核呼吸病组组间比较差异无统计学意义(P>0.05)。经相关性检验,活动性肺结核组IFN-γ及IP-10水平呈正相关关系(r=0.154,P=0.004)。IFN-γ与IP-10 2个指标诊断活动性肺结核的ROC曲线下面积为0.927和0.975。IFN-γ和IP-10诊断活动性肺结核的灵敏度分别为90.5%和95.9%,特异度分别为92.3%和97.1%。以对照组和非结核呼吸病组为对照,血清IP-10检测活动性肺结核的敏感性和特异性(95.5%、97.1%)高于血清T-SPOT的敏感性和特异性(75.4%、72.6%),同时也高于IFN-γ的敏感性和特异性(90.5%,92.3%)。结论采用ELISA检测血清IP-10水平,操作方法简便,质量控制明确,敏感性和特异性均呈较高水平,今后可进一步扩大样本证实其敏感性与特异性,为活动性肺结核的快速诊断提供理论依据。
Objective To investigate the diagnostic value ofγ-interferon-inducible protein 10（IP-10）in the blood of patients with active pulmonary tuberculosis.Methods A total of 72 patients with active pulmonary tuberculosis admitted to the First Affiliated Hospital of Xinjiang Medical University from January 2017 to December 2017 were enrolled in this study.40 patients with non-tuberculosis lung disease（non-tuberculosis respiratory disease group）were recruited from the same hospital.In the same period,40 hospitalized patients with lung diseases such as pneumonia,lung cancer,and emphysema were admitted to the hospital and selected as control group.In the control group,30 patients were from the same period as the health examiners from our hospital.ELISA was used to detect IP-10 and IFN-γof the 3 groups.The actual operation was performed according to the instructions.Blood T-SPOT was performed according to the T-SPOT TB kit protocol.Results There was significant difference of IP-10 and IFN-γlevels between these groups（P 0.05）.The levels of IP-10 and IFN-γin the active tuberculosis group was significantly higher than those in the non-tuberculosis respiratory disease group and the healthy control group,and the difference was statistically significant（P 0.05）.There was no significant difference between the healthy control group and the non-tuberculosis respiratory disease group（P 0.05）.After correlation test,there was a positive correlation between IFN-γand IP-10 levels in the active tuberculosis group（r=0.154,P =0.004）.The area under the ROC curve for the diagnosis of active pulmonary tuberculosis with IFN-γand IP-10 was0.927 and 0.975.The sensitivity of IFN-γand IP-10 in the diagnosis of active tuberculosis were 90.5%and95.9%,respectively.And the specificities were 92.3% and 97.1%,respectively.The sensitivity（95.5%,97.1%）of IP-10 detection was higher than that of blood T-SPOT（75.4%,72.6%）,and it was also higher than the sensitivity and specificity（90.5%,92.3%）of IFN-γ.Conclusion Usin
作者
张迪
孟存仁
王家路
ZHANG Di;MENG Cunren;WANG Jialu(Laboratory Medicine Diagnostic Center,The First AJfiliated Hospital,Xinjiang Medical University,Urumqi 830054,China)
出处
《新疆医科大学学报》
CAS
2018年第9期1095-1098,共4页
Journal of Xinjiang Medical University
基金
新疆维吾尔自治区自然科学基金(2017D01C314)
