摘要
目的:为了指明海洋植物红树次生代谢产物研究方向,丰富来源于桐花树的化合物类型,深入研究了桐花树(Aegiceras cornicnlatnm)的可再生部位胚轴的化学成分。方法:采用多种常压柱层析(正相硅胶、凝胶sephadex LH-20)和高效液相色谱法进行分离纯化桐花树胚轴中化合物,运用现代波谱学方法进行结构解析。结果:从桐花树的可再生部位胚轴中共分离得到14个化合物,分别鉴定为:4-烯丙基-2,6-二甲氧基苯基-葡萄糖苷(1)、3,4-二咖啡奎尼酸(2)、3,4,5-三咖啡奎尼酸(3)、3.,4.,5,7-四羟基黄酮-3-O-β-D-葡萄糖苷(4)、3,5,7-三羟基-2-(4.-羟苯基)-4-酮(5)、2-(3.,4.-二羟苯基)-5,7-二羟基-4-酮(6)、5,7-二羟基-异苯唑呋喃-7-O-β-D-吡喃葡萄糖苷(7)、5,7-二羟基-2-(4.-羟苯基)-4-酮(8)、2-(3.,4.-二羟苯基)-3,5,7-三羟基-4-酮(9)、3,5,8-三羟基-2-(3.,4.,5.-三苯羟基)-4-酮(10)、2-(2.,3.-二羟苯基)-3,5,8-三羟基-4-酮(11)、3,5-二羟基-4-甲基-2-(3.,4.,5.-三羟基-6.-(羟甲基)-四氢-2H-吡喃-2-基)-苯甲酸(12)、豆甾醇3-O-β-D-葡萄糖苷(13)、4-(4'-羟基-3'-甲氧基苯基)-2-丁酮(14)。结论:除化合物9外,其他化合物均为首次在桐花树中分离鉴定得到,研究丰富了桐花树化合物类型,提高了桐花树化合物的数量,为红树次生代谢产物研究指明了新的方向,为桐花树可再生部位胚轴的应用奠定了坚实基础。
This paper was aimed to study chemical constituents from hypocotyls of Mangrove Aegiceras corniculatum.Chemical constituents were isolated and purified by pressure column chromatography（normal phase silica gel, sephadex LH-20 gel） and HPLC. The structures were identified by spectroscopic analysis and compared with literatures. The results showed that 14 compounds were isolated and identified as 4-allyl-2,6-dimethoxyphenyl glucoside（1）, 3,4-dicaffeoylquinic acid（2）, 3,4,5-tricaffeoylquinic acid（3）, 3.,4.,5,7-tetrahydroxyflavone-3-O-β-D-glucoside（4）, 3,5,7-trihydroxy-2-（4.-hydroxyphenyl）-4-one（5）, 2-（3.,4.-dihydroxyphenyl）-5,7-dihydroxychroman-4-one（6）, 5,7-dihydroxy-isobenzofuran-7-O-β-D-glucopyranoside（7）, 5,7-dihydroxy-2-（4.-hydroxyphenyl）-4-one（8）, 2-（3.,4.-dihydroxyphenyl）-3,5,7-trihydroxy-4-one（9）, 3,5,8-trihydroxy-2-（3.,4.,5.-trihydroxyphenyl）-4-one（10）, 2-（2.,3.-dihydroxyphenyl）-3,5,8-trihydroxy-4-one（11）, 3,5-dihydroxy-4-methyl-2-（3.,4.,5.-trihydroxy-6.-（hydroxymethyl）tetrahydro-2 H-pyran-2-yl） benzoic acid（12）, stigmast-3-O-β-D-glucopyranosyl（13）, 4-（4.-hydroxy-3.-methoxyphenyl） 2-butanone（14）. It was concluded that all compounds were isolated from this plant for the first time except compound（9）. This study enriched the chemical composition types and increased the chemical composition amount of Aegiceras corniculatum. It showed a new direction in the study of secondary metabolites from Mangrove. This study also laid a solid foundation for the application of hypocotyl from renewable parts of Aegiceras corniculatum.
作者
易湘茜
徐普
邓家刚
高程海
Yi Xiangxi;Xu Pu;Deng Jiagang;Gao Chenghai(School of Pharmaceutical Sciences,Guangxi University of Chinese Medicine,Nanning 530020,China;Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica,Nanning 530020,China;Guangxi Scientific Research of Traditional Chinese Medicine,Nanning 530020,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2018年第5期684-689,共6页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
广西壮族自治区科学技术厅广西重点实验室建设项目(16-380-29):广西中药药效研究重点实验室
负责人:邓家刚
广西壮族自治区科学技术厅广西科技计划项目(桂科合15104001-11):基于高内涵筛选平台的京族习用海洋药物抗癌新药发现关键技术合作研究
负责人:邓家刚
关键词
桐花树
胚轴
化学成分
结构鉴定
Aegiceras corniculatum
hypocotyl
chemical composition
structural identification
