摘要
目的:建立清热解毒冲洗液的质量控制方法。方法:采用薄层色谱法对方中当归、苦参、大黄、赤芍进行定性鉴别,采用高效液相色谱法测定清热解毒冲洗液中大黄有效成分大黄酸、大黄素、大黄酚的含量。结果:薄层色谱特征斑点清晰,专属性强,阴性对照无干扰;高效液相色谱法测定大黄酸在0.056~0.56μg范围内线性关系良好(r=0.999 8,n=5),平均回收率为98.47%(RSD=0.77%,n=5);大黄素在0.179~1.79μg范围内线性关系良好(r=0.999 7,n=5),平均回收率为98.58%(RSD=0.51%,n=5);大黄酚在0.035~0.35μg范围内线性关系良好(r=0.999 0,n=5),平均回收率为98.63%(RSD=0.66%,n=5)。结论:该方法简便可靠、专属性强、重复性好,可用于该制剂的质量控制。
Objective：To establish the method for quality control of heat-clearing detoxifying flush fluid.Methods：DangGui（Angelica sinensis）,kuh-seng,Da Huang（Rheum palmatum L.） and Chi Shao（Radix Paeoniae Rubra）in the prescription were identified qualitatively by thin-layer chromatography（TLC）,the contents of the active ingredients of Da Huang including chrysophanic acid,emodin and rheic acid were measured by HPLC.Results：TLC was featured by clear spots,strong specificity,no interference with negative controls; the results of HPLC showed that chrysophanic acid had good linear relationship in the range between 0.056~0.56 μg（r=0.999 8,n=5）,average recovery rate was 98.47%（RSD=0.77%,n=5）; emodin presented good linear relationship in the range between 0.179~1.79μg（r=0.999 7,n=5）,average recovery rate was 98.58%（RSD=0.51%,n=5）; rheic acid demonstrated good linear relationship in the range between 0.035~0.35 μg（r=0.999 0,n=5）,average recovery rate was 98.63%（RSD=0.66%,n=5）.Conclusion：The method,simple,convenient,reliable,specific and reproducible,could be used for quality control of the preparation.
作者
焦正花
李文萍
赵凤菊
禚君
JIAO Zhenghua;LI Wenping;ZHAO Fengju;ZHUO Jun(Gansu Provincial Hospital of Traditional Chinese Medicine,Lanzhou 730050,China;Gansu Provincial Cancer Hospital)
出处
《西部中医药》
2018年第8期16-19,共4页
Western Journal of Traditional Chinese Medicine
基金
甘肃省中医药管理局科研课题(编号GZK-2016-12)
关键词
清热解毒冲洗液
薄层色谱
高效液相
质量控制
heat-clearing detoxifying flush fluid
thin-layer chromatography
HPLC
quality standard
