摘要
采用双因素完全随机区组试验探讨耐性真菌联合香根草修复铅（Pb）、镉（Cd）复合污染土壤效应.其中A因素为加菌量,设置0、1、3、5 g共4个水平,菌株代号Q7.B因素为铅镉添加量,设置Cd0Pb0、Cd10Pb400、Cd20Pb600、Cd30Pb800、Cd50Pb1200、Cd80Pb1800（单位为mg/kg）6种不同铅镉浓度水平.结果表明,添加耐性真菌可显著提高香根草生物量,1、3、5 g加菌量处理可分别使香根草总干重增加量达到41.9%、74.9%、71.7%.耐性真菌可促进香根草地上部与地下部对铅镉的富集,Cd^2＋80 mg/kg、Pb^2＋1 800 mg/kg处理下,1、3、5 g加菌量处理香根草对铅富集量比不加菌处理,地上部分别升高了120.6%、265.4%、242.9%,地下部分别升高了110.3%、278.2%、266.2%;对镉而言,地上部镉含量分别升高了113.2%、238.3%、217.3%,地下部分别升高了103.1%、298.8%、274.4%.加菌可强化香根草对铅镉污染土壤的修复效果,且3 g加菌量强化香根草修复效果要优于1 g和5 g加菌量处理.综上,将耐性真菌与重金属富集植物香根草在不同铅镉复合污染浓度下构建联合修复体系,显示了真菌对香根草修复铅镉污染土壤的显著强化效应.
A two-factor randomized complete block experiment was used to explore the remediation by a plant-microorganism combination on soils contaminated by lead（Pb） and cadmium（Cd）. Factor A was the amount of fungi, for which four values were considered, namely, 0, 1, 3, and 5 g. Factor B was the level of contamination by lead and cadmium, for which six values were considered, namely, Cd0 Pb0, Cd10 Pb400, Cd20 Pb600, Cd30 Pb800, Cd50 Pb1200, and Cd80 Pb1800（data in units of mg/kg）. The results showed that the resistant fungi promoted the growth of vetiver（Vetiveria zizanioides）. At weights of 1, 3, and 5 g, the resistant fungi increased the biomass of vetiver by 41.9%, 74.9%, and 71.7% respectively. The resistant fungi stimulated the absorption of lead and cadmium by both the aerial and underground parts of vetiver. In the presence of 80 mg/kg of Cd^2＋and 1 800 mg/kg of Pb^2＋, the contents of lead in the aerial parts of vetiver were increased by 120.6%, 265.4%, and 242.9%, while the lead content in the underground parts were increased by 110.3%, 278.2%, and 266.2%, after the addition of 1 g, 3 g, and 5 g of fungi, respectively. The content of cadmium in the aerial parts increased by 113.2%, 238.3%, and 217.3%, while the content of cadmium in the underground parts increased by 103.1%, 298.8%, and 274.4%, after the addition of 1 g, 3 g, and 5 g of fungi, respectively. The addition of fungi strengthened the effect of V. zizanioides to remediate soils contaminated by lead and cadmium, and the remediation after the addition of 3 g of fungi was better than that after treatment with 1 g and 5 g of fungi. The combination of resistant fungi and the heavy metal enrichment plant, vetiver, under different concentrations of lead and cadmium showed that the fungi had a significant effect on the remediation of soils contaminated by lead and cadmium.
作者
黄成涛
黄位权
史鼎鼎
徐少慧
黄智刚
蒋代华
HUANG Chengtao;HUANG Weiquan;SHI Dingding;XU Shaohui;HUANG Zhigang;JIANG Daihua(Agricultural College,Guangxi University,Nanning 530004,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2018年第4期901-907,共7页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金项目(41661076)
广西自然科学基金项目(2014GXNSFAA118287)资助~~
关键词
耐性真菌
重金属污染
香根草
植物-微生物联合修复
metal-resistant fungi
heavy metal contamination
Vetiveria zizanioides
plant-microorganism combined remediation
