摘要
为利用近红外光谱(NIRS)技术建立一种用于快速无损检测甜玉米种子发芽率、发芽指数、活力指数等活力指标的新方法,选取8个代表性甜玉米品种种子为试材,分别将每品种分成5份(共40份样品);将所有样品置于50℃干燥箱内,分别处理1、2、3、4、5 d后取出,进行生理检测和光谱采集;利用偏最小二乘法回归(PLSR)建立甜玉米种子活力相关指标的定量模型。结果表明,发芽率定量分析模型的性能参数校正集相关系数(Rc)、预测集相关系数(Rp)、交叉验证相关系数(Rcv)、校正集均方根误差(RMSEC)、验证集均方根误差(RMSEP)、交叉验证均方根误差(RMSECV)分别为0.8952、0.8605、0.8503、6.6490、6.6270、7.9091。发芽指数的PLSR模型参数Rc、Rp、Rcv、RMSEC、RMSEP、RMSECV分别为0.9710、0.9366、0.9427、7.4373、24.7921、10.5003。活力指数PLSR模型参数分别为0.9109、0.8962、0.8711、0.8674、0.9489、1.0393。综上,NIRS技术简便、快速、准确可靠,适用于甜玉米发芽率、发芽指数、活力指数等多参数的快速评价。本研究结果为高活力玉米种子筛选提供了一种新的快速分析技术。
The purpose of this study was to establish a new method for rapid and nondestructive detection of sweet corn seed vigor（including germination percentage,germination index and vigor index） by using near infrared spectroscopy.Eight sweet corn varieties were used as test materials and the seeds of each variety were divided into 5 sets with total of40 samples. All samples were oven-dried at 50℃,then one set of the sweet corn seeds of each variety was taken out at1 day,2 days,3 days,4 days and 5 days after drying for the near infrared spectroscopy detection and investigation of biological index. A quantitative model of maize correlation index was established by using the partial least square regression（PLSR） method. The results showed that the correlation coefficient for calibration（Rc）,correlation coefficient for prediction（Rp）,correlation coefficient for cross validation（Rcv）,root mean square error for calibration（RMSEC）,root mean square error for prediction（RMSEP） and root mean square error for cross validation（RMSECV）were 0. 8952,0. 8605,0. 8503,6. 6490,6. 6270,7. 9091 for germination percentage,respectively. The parameters of PLSR model of germination index,Rc,Rp,Rcv,RMSEC,RMSEP and RMSECV were 0. 9710,0. 9366,0. 9427,7. 4373,24. 7921 and 10. 5003,respectively. The relative parameters of the vigor index were 0. 9109,0. 8962,0. 8711,0. 8674,0. 9489 and 1. 0393,respectively. The method was simple,fast,accurate and reliable,and was suitable for rapid and nondestructive evaluation of sweet corn germination rate,germination index,vigor index and other seed germination indexes. This study provides a new rapid method for high vigor corn seeds screening with high in the future.
作者
李武
李妍
李高科
高磊
陈敏忠
卢爵广
胡建广
刘建华
LI Wu;LI Yan;LI Gaoke;GAO Lei;CHEN Minzhong;LU Jueguang;HU Jianguang;LIU Jianhua(Guangdong Provincial Key Laboratory of Crop Genetic Improvement/Crops Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou,Guangdong 510640;Institute of Pharmaceutical Sciences,Shandong Academy of Medical Sciences,Jinan,Shandong 250012;Zhanjiang Branch Courts of Guangdong Academy of Agricultural Sciences/Zhanjiang Academy of Agricultural Sciences,Zhanjiang,Guangdong 524094)
出处
《核农学报》
CAS
CSCD
北大核心
2018年第8期1611-1618,共8页
Journal of Nuclear Agricultural Sciences
基金
广东省科技计划项目(2015B020202006
2016B020233004
2016A030303030
2016A020210030
2017B090907023)
广东省省级现代种业提升工程项目“广东省特色经济作物重要品质性状改良及优异种质创新研究”
关键词
甜玉米
种子活力
近红外光谱
高温老化
sweet corn
seed vigor
near infrared spectroscopy
high temperature stress
