摘要
目的分析珠海市男男性行为(MSM)人群HIV-1流行亚型的分布与变化。方法收集珠海市2011年-2016年386例MSM人群HIV-1抗体阳性血清标本,提取病毒RNA,套式PCR对HIV-1 pol基因进行扩增、测序亚型序列分析。结果 CRF_07BC和CRF_01AE基因亚型占主要地位,分别为155例(占48.87%)和99例(占32.03%);其次是CRF55_01B亚型30例(占9.71%),同时也发现了B亚型11例(占3.56%)、CRF59_01B亚型10例(占3.24%)、CRF_08BC亚型3例(占0.97%)、CRF65_01B亚型3例(占0.97%)、CRF_01C亚型2例(占0.65%)。系统进化树显示CRF_07BC内部未见明显的分簇,CRF_01AE内部可分为两簇。结论珠海市MSM人群HIV-1基因亚型以CRF_01AE和CRF_07BC为主,其他多种基因型共同流行的现象,应加强该人群的干预和监测力度。
Objective To investigate the distribution and changes of HIV-1 subtype prevalent in men who have sex with men（MSM） in Zhuhai. Methods Serum samples of positive HIV-1 from 386 MSM in Zhuhai were collected from 2011 to 2016.Virus RNA was extracted and nested PCR was used to amplify and sequence subtypes of HIV-1 pol gene. Results The highest proportions of the subtypes were CRF_07 BC（48. 87%,n = 155） and CRF_01 AE（32. 03%,n = 99）,followed by CRF55_01 B（9. 71%,n =30） and subtype B（3. 56%,n =11）,CRF59_01 B（3. 24%,n = 10）,CRF_08 BC（0. 97%,n = 3）,CRF65_01 B（0. 97%,n = 3）,CRF_01 C（0. 65%,n = 2）. The phylogenetic tree shows that there is no obvious clustering inside CRF_07 BC,and CRF_01 AE can be divided into two clusters. Conclusion CRF_07 BC and CRF_01 AE are the major epidemic HIV-1 infective strains among MSM in Zhuhai,and other recombinant subtypes are also prevalent. Intervention and monitoring should be enhanced among this MSM group.
作者
林毅雄
魏泉德
张丽荣
京舒娉
周兰兰
周毅
LIN Yi-xiong;WEI Quan-de;ZHANG Li-rong;JING Shu-ping;ZHOU Lan-lan;ZHOU Yi(Zhuhai Center for Disease Control and Prevention,Zhuhai,Guangdong 519000,China)
出处
《中国卫生检验杂志》
CAS
2018年第15期1803-1805,共3页
Chinese Journal of Health Laboratory Technology
基金
广东省医学科研基金项目(A2017491)
关键词
人类免疫缺陷病毒
男男性行为
基因亚型
Human immunodeficiency virus
Men who have sex with men
Subtypes
